000			03933nam a2200397 i 4500
999			_c396572 _d396572
001			396572
003			ES-MaUEC
005			20231211085647.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			211221s2022 xxua o |||| 0|eng d
020			_a9781071619049
024	7		_a10.1007/978-1-0716-1904-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH605 _b2022 EB
245	0	0	_aMitosis : _bMethods and Protocols _cedited by Edward H. Hinchcliffe
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 256 páginas) _b55 ilustraciones, 44 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2415
505	0		_aUse of Echinoderm Gametes and Early Embryos for Studying Meiosis and Mitosis -- Methods for Investigating Cell Division Mechanisms in C. elegans -- Visualizing the Dynamics of Cell Division by Live Imaging Drosophila Larval Brain Squashes -- Imaging Chambers for Arabidopsis Seedlings for Mitotic Studies -- Live Fluorescence Imaging of Chromosome Segregation in Cultured Cells -- Staining of the Actin Cytoskeleton during Cell Division in Budding Yeast and Mammalian Cells -- Synchronizing Mammalian Cells for Mitotic Analysis of the Localization of Survivin -- Using Microinjection of Mammalian Cultured Cells to Study Cell Division -- In Vitro and In Vivo Approaches to Study Kinetochore-Microtubule Attachments during Mitosis -- Quantifying Changes in Chromosome Position to Assess Chromokinesin Activity -- Chemical Biology of Mitotic Spindle Assembly Motors -- Deploying Kinase Inhibitors to Study Pediatric Gliomas -- Identifying Cyclin A/Cdk1 Substrates in Mitosis in Human Cells -- Degron Tagging Using mAID and SMASh Tags in RPE-1 Cells -- Centromere Tension Measurement in Budding Yeast Mitosis -- Simulating Dynamic Chromosome Compaction: Methods for Bridging In Silico to In Vivo -- Using FLIM-FRET for Characterizing Spatial Interactions in the Spindle -- Imaging Mitosis with Lattice LightSheet.
520			_aThis detailed volume collects a selection of key techniques for studying cell division, representing multiple model systems and varied scales of approach. Over the past 20 years, a series of revolutions in experimental molecular biology, including chimeric fluorescent protein expression, multiple advanced modes of quantitative microscopy, and array of small molecule inhibitors, proteomic profiling, and gene silencing/manipulation/analysis, has advanced the mitosis field to a point where single cell biology not only allows for imaging/localization studies, but also for quantitative analysis and sequencing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitosis: Methods and Protocols provides a repository of techniques and approaches for those working in the field as well as a working resource for those venturing into the study of mitosis for the first time.
988			_aSpringer_Protocols_2022
650		7	_2embne _9689279 _aDivisión celular
776	0	8	_iPrinted edition: _z9781071619032
776	0	8	_iPrinted edition: _z9781071619056
776	0	8	_iPrinted edition: _z9781071619063
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1904-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI