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008			211215s2022 xxua o |||| 0|eng d
020			_a9781071618479
024	7		_a10.1007/978-1-0716-1847-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQL822 _b2022 EB
245	0	0	_aCraniofacial Development : _bMethods and Protocols _cedited by Sebastian Dworkin
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XII, 313 páginas) _b94 ilustraciones, 73 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2403
505	0		_a1. Using Caenorhabditis elegans as a model for mechanistic insights of craniofacial development -- Wholemount In-situ Hybridisation (WISH) in Zebrafish Embryos to Analyse Craniofacial Developmen -- Elucidationof Gene Expression Patterns in The Craniofacial Tissues Of Mouse Embryos By Whole-MountIn Situ Hybridization -- Visualizationof the Cartilage and Bone Elements in The Craniofacial Structures by Alcian blue and Alizarin Red Staining -- Methodology for Free-Floating Organ Culture of Mid-Gestation Maxillary Primordial Tissue -- Methods of Palate Culture in Later Palatogenesis: Elevation, Horizontal Outgrowth, and Fusion -- Single cell RNA-seq: Cell Isolation and Data Analysis -- Generating Zebrafish RNA-less Mutant Alleles by Deleting Gene Promoters with CRISPR/Cas9 -- Craniofacial Phenomics: Three-Dimensional Assessment of the Size and Shape of Cranial and Dentofacial Structures -- Micro-CT-based Bone Microarchitecture Analysis of the Murine Skull -- Characterization of mammalian in vivo enhancers using mouse transgenesis and CRISPR genome editing -- Histological Techniques for Sectioning Bones of the Vertebrate Craniofacial Skeleton -- Engineering Epithelial-Mesenchymal Microtissues to Study Cell-Cell Interactions in Development -- Ex Vivo Culture of Human Cranial Suture Cells -- Scaffolds for Use in Craniofacial Bone Regeneration -- Mandible Explant Assay for The Analysis of Meckel's Cartilage Development -- High Resolution Histology for Craniofacial Studies On Zebrafish and Other Teleost Models -- Live Imaging the Dynamics Of Mammalian Neural Crest Cell Migration -- Salivary Gland Development In Culture -- Antenatal Ultrasound Imaging for Analysis of Human Craniosynostosis.
520			_aThis volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (c. elegans, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and in vivo cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using in-situ hybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR/Cas9-mediated deletion. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Craniofacial Development: Methods and Protocols aims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques. .
988			_aSpringer_Protocols_2022
650		7	_2embne _9139947 _aCráneo
650		7	_2embne _9675734 _aHuesos faciales
650		7	_2embne _9671711 _aOsteología
776	0	8	_iPrinted edition: _z9781071618462
776	0	8	_iPrinted edition: _z9781071618486
776	0	8	_iPrinted edition: _z9781071618493
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1847-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI