000			04175nam a2200397 i 4500
999			_c396560 _d396560
001			396560
003			ES-MaUEC
005			20230714111704.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			211206s2022 xxua o |||| 0|eng d
020			_a9781071619445
024	7		_a10.1007/978-1-0716-1944-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR181 _b2022 EB
245	0	0	_aImmune Receptors : _bMethods and Protocols _cedited by Jonathan Rast, Katherine Buckley
250			_a2nd edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 268 páginas) _b43 ilustraciones, 31 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2421
505	0		_aMicropatterned Ligand Arrays to Investigate Spatial Regulation of Cellular Signaling Initiated by Clustered Fc Receptors -- Low-Cost and Cell-Surface-Mimic Analysis of Ligand Interactions of Biotinylated Immune Receptors Using Surface Plasmon Resonance -- Analysis of the Sub-Cellular Localization of NLRs Upon Shigella flexneri Infection Exemplified by NOD1 -- Generation of VNAR Libraries from Immunized Sharks and Selection of Target-Specific Clones -- Identification of Glycan-Specific Variable Lymphocyte Receptors using Yeast Surface Display and Glycan Microarrays -- Cell Aggregation Assays for Homophilic Interactions between Cell Surface Protein -- Measuring Mutator Enzyme Activity using an E. coli-Based Colony Formation Assay -- Lamprey Variable Lymphocyte Receptor Monoclonal Antibodies for Whole Cell Surface Antigens -- A Role for Secreted Immune Effectors in Microbial Biofilm Formation Revealed by Simple In Vitro Assays -- Assessment of Scavenger Receptor Cysteine Rich Domain Binding to Bacteria -- Bioinformatics Approaches for Analyzing Multigene Families Encoding Immune Receptors -- Production and Characterization of SIV-Specific CAR/CXCR5 T Cells -- Engineering IgG1 Fc Domains that Activate the Complement System -- An Imaging Flow Cytometry Protocol for Studying Immunoregulatory Receptor-Mediated Regulation of Phagocytosis -- Single Cell Sequencing for Everybody -- Exploring the Diversity of the B-Cell Receptor Repertoire Through High-Throughput Sequencing -- Fetal Thymic Organ Culture (FTOC) Optimized for Gamma-Delta T Cell Studies.
520			_aThis volume explores immune cell receptors that are used in the detection of microbes, either by binding directly to non-self molecules or through indirectly sensing microbe-associated cellular disturbances. The chapters in this book cover methods for studying receptor-ligand interactions at both molecular and cellular levels; methods to create and characterize novel antibody reagents; and methods to characterize the molecular processes that lead to adaptive receptor maturation. This book also contains chapters that look at high-throughput strategies that describe the diversity of immune receptors and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Immune Receptors: Methods and Protocols, Second Edition is a valuable resource for scientists and researchers interested in learning more about this developing field. .
988			_aSpringer_Protocols_2022
650		7	_2embne _9138330 _aInmunología
776	0	8	_iPrinted edition: _z9781071619438
776	0	8	_iPrinted edition: _z9781071619452
776	0	8	_iPrinted edition: _z9781071619469
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1944-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI