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008			211206s2022 xxua o |||| 0|eng d
020			_a9781071619087
024	7		_a10.1007/978-1-0716-1908-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH588 .S83 _b2022 EB
245	0	0	_aHuman Naïve Pluripotent Stem Cells _cedited by Peter Rugg-Gunn
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (X, 288 páginas) _b66 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2416
505	0		_aDeriving Human Naïve Embryonic Stem Cell Lines from Donated Supernumerary Embryos using Physical Distancing and Signal Inhibition -- Induction of Human Naïve Pluripotency Using 5i/L/A Medium -- Induction of Human Naïve Pluripotency Using Chemical Resetting -- Induction of Human Naïve Pluripotent Stem Cells from Somatic Cells -- Using Microfluidics to Generate Human Naïve and Primed Pluripotent Stem Cells -- Maintenance of Human Naïve Pluripotent Stem Cells -- Generating Trophoblast Stem Cells from Human Naïve Pluripotent Stem Cells -- Differentiation and Expansion of Human Extra-Embryonic Endoderm Cell Lines from Naïve Pluripotent Stem Cells -- Capacitation of Human Naïve Pluripotent Stem Cells -- Generation of Pericytic-Vascular Progenitors from Tankyrase/PARP-Inhibitor-Regulated Naïve (TIRN) Human Pluripotent Stem Cells -- Profiling DNA Methylation in Human Naïve Pluripotent Stem Cells -- Chromatin Profiling of Human Naïve Pluripotent Stem Cells -- Mapping Chromatin Accessibility in Human Naïve Pluripotent Stem Cells using ATAC-seq -- Transcriptomic Analysis of Human Naïve and Primed Pluripotent Stem Cells -- Study of X Chromosome Activity Status in Human Naïve Pluripotent Stem Cells using RNA-FISH -- Flow Cytometry Analysis of Cell-Surface Markers to Identify Human Naïve Pluripotent Stem Cells -- Characterizing the Genetic Stability of Human Naïve and Primed Pluripotent Stem Cells.
520			_aThis volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naïve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate naïve hPSC lines; methods to differentiate naïve hPSCs into specialized cell types; and techniques to characterize naïve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Human Naïve Pluripotent Stem Cells: Methods and Protocols is a valuable resource for novice and expert researchers who are looking to learn more or expand their research in this developing field.
988			_aSpringer_Protocols_2022
650		7	_2embne _9160528 _aCélulas madre
776	0	8	_iPrinted edition: _z9781071619070
776	0	8	_iPrinted edition: _z9781071619094
776	0	8	_iPrinted edition: _z9781071619100
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1908-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI