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008			211202s2022 xxua o |||| 0|eng d
020			_a9781071619483
024	7		_a10.1007/978-1-0716-1948-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR183.6 _b2022 EB
245	0	0	_aImmunohistochemistry and Immunocytochemistry : _bMethods and Protocols _cedited by Luis Del Valle
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (X, 286 páginas) _b50 ilustraciones, 43 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2422
505	0		_aIntroduction to Immunohistochemistry. From Evolving Science to Timeless Art -- Immunohistochemistry in Historical Perspective. Knowing the Past to Understand the Present -- Sample Preparation & Fixation for Histology and Pathology -- Tissue Processing -- Antigen Retrieval and Signal Amplification -- Primary Antibody Selection and Blocking Techniques for Immunohistochemistry -- Probes for Fluorescent Visualization of Specific Cellular Organelles -- Enzymatic Immunohistochemistry -- Fluorescent Immunohistochemistry -- Double Labeling Fluorescent Immunocytochemistry -- Multiplexing and Spectral Microscopy -- In situ Hybridization (Fluorescent and Enzymatic) -- Proximity Ligation Assay (PLA) -- Immunohistochemistry, Quality control and Principles of Validation in the Central Nervous System -- Culture and Phenotyping of Glial Cell Cultures, Gliospheres and Neurospheres -- ER+ Breast Cancer Mammosphere Formation and Analysis -- Detection of Circulating and Tissue Myeloid-Derived Suppressor Cells (MDSCs) by Flow Cytometry -- Comet Assay for the Detection of Single and Double Strand DNA Breaks -- Fluorescent-Activated Cell Sorting (Flow Cytometry).
520			_aThis volume provides a comprehensive reference guide for researchers to study the applications of labeled antibodies. Chapters guide reader through the the and practice of immunohistochemistry, immunocytochemistry and immunofluorescence techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and useful tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunohistochemistry and Immunofluorescence: Methods and Protocols aims to be a useful practical guide to scientists to help further their study in this field.
988			_aSpringer_Protocols_2022
650		7	_2embne _9163078 _aInmunohistoquímica
776	0	8	_iPrinted edition: _z9781071619476
776	0	8	_iPrinted edition: _z9781071619490
776	0	8	_iPrinted edition: _z9781071619506
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1948-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI