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020 _a9781071618431
024 7 _a10.1007/978-1-0716-1843-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP751
_b2022 EB
245 0 0 _aMembrane Lipids :
_bMethods and Protocols
_cedited by Charles G. Cranfield
250 _a1st edition 2022
264 1 _aNew York, NY
_bSpringer International Publising
_c2022
300 _a1 recurso en línea (XI, 300 páginas)
_b145 ilustraciones, 136 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2402
505 0 _aMethods for Forming Giant Unilamellar Fatty Acid Vesicles -- Preparing Ion Channel Switch Membrane-Based Biosensors -- Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely-Tethered Lipid Bilayer -- Electrochemical Impedance Spectroscopy as a Convenient Tool to Characterize Tethered Bilayer Membranes -- Measuring Voltage-Current Characteristics of Tethered Bilayer Lipid Membranes to Determine the Electro-Insertion Properties of Analytes -- Measuring Activation Energies for Ion Transport Using Tethered Bilayer Lipid Membranes (tBLMs) -- Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation Conductance Measures of Tethered Bilayer Lipid Membranes -- De-Insertion Current Analysis of Pore-Forming Peptides and Proteins Using Gold Electrode-Supported Lipid Bilayer -- Drug Meets Monolayer: Understanding the Interactions of Sterol Drugs with Models of the Lung Surfactant Monolayer Using Molecular Dynamics Simulations -- Establishing a Lipid Bilayer for Molecular Dynamics Simulations -- Initiating Coarse-Grained MD Simulations for Membrane-Bound Proteins -- Small Angle Neutron Scattering of Liposomes: Sample Preparation to Simple Modeling -- Time-Resolved SANS to Measure Monomer Inter-Bilayer Exchange and Intra-Bilayer Translocation -- Identifying Membrane Lateral Organization by Contrast-Matched Small Angle Neutron Scattering -- Using refnx to Model Neutron Reflectometry Data from Phospholipid Bilayers -- Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS) to Probe Interfacial Water in Floating Bilayer Lipid Membranes (fBLMs) -- Manipulation of Lipid Membranes with Thermal Stimuli -- Analyzing Morphological Properties of Early-Stage Toxic Amyloid β Oligomers by Atomic Force Microscopy -- Formation and Nanoscale Characterization of Asymmetric Supported Lipid Bilayers Containing Raft-Like Domains -- Rapid FLIM Measurement of Membrane Tension Probe Flipper-TR -- Bacterial Dye Release Measures in Response to Antimicrobial Peptides -- Quantitative Measurements of Membrane Lipid Order in Yeast and Fungi.
520 _aThis detailed book explores examples of current in vitro and in silico techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane models in various research settings, the volume continues with electrical impedance spectroscopy strategies and methods to identify how ions and proteins interact with model lipid bilayers, guidance on lipid bilayer in silico molecular dynamics modeling, novel techniques to explore lipid bilayer characteristics using neutron scattering, IR spectroscopy, and atomic force microscopy (AFM), as well as unique fluorescence techniques. Written in the highly successful Methods in Molecular Biology series style, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Membrane Lipids: Methods and Protocols serves as an ideal guide for researchers seeking to further investigate the often complicated world of lipid membrane biophysics.
988 _aSpringer_Protocols_2022
650 7 _2embne
_9142933
_aLípidos
650 7 _2embne
_9139103
_aBiología molecular
776 0 8 _iPrinted edition:
_z9781071618424
776 0 8 _iPrinted edition:
_z9781071618448
776 0 8 _iPrinted edition:
_z9781071618455
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1843-1
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b07/2023
_dz
_eb
_zSI