000			04245nam a2200397 i 4500
999			_c396555 _d396555
001			396555
003			ES-MaUEC
005			20230714094228.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			211130s2022 xxua o |||| 0|eng d
020			_a9781071617168
024	7		_a10.1007/978-1-0716-1716-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2022 EB
245	0	0	_aProtein Folding : _bMethods and Protocols _cedited by Victor Muñoz
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 419 páginas) _b156 ilustraciones, 78 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2376
505	0		_aMutational Analysis of Protein Folding Transition States: Phi-values -- Engineered Metal-Binding Sites to Probe Protein Folding Transition States: Psi Analysis -- Site-Specific Interrogation of Protein Structure and Stability -- Purification and Handling of the Chaperonin GroEL -- ­Folding free energy surfaces from Differential Scanning Calorimetry -- Fast Folding Kinetics using Nanosecond Laser-Induced Temperature Jump Methods7. Measurement of Submillisecond Protein Folding using Trp Fluorescence and Photochemical Oxidation -- Native State Hydrogen Exchange-Mass Spectrometry Methods to Probe Protein Folding and Unfolding -- Multi-Probe Equilibrium Analysis of Gradual (Un)Folding Processes -- Nmr Analysis Of Protein Folding Interaction Networks -- NMR Relaxation Dispersion Methods for the Structural and Dynamic Analysis of Quickly Interconverting, Low-Populated Conformational Sub-States -- Native State Hydrogen Exchange-Mass Spectrometry Methods to Probe Protein Folding and Unfolding -- Single-Molecule Fluorescence Spectroscopy Approaches for Probing Fast Biomolecular Dynamics and Interactions -- Theory and Analysis of Single-Molecule FRET experiments -- Mechanochemical Evolution Of Disulfide Bonds In Proteins -- Coarse-Grained Simulations of Protein Folding: Bridging Theory and Experiments -- Analysis of Molecular Dynamics Simulations of Protein Folding -- Atomistic Simulations of Thermal Unfolding -- Molecular Simulations of Intrinsically Disordered Proteins and their Binding Mechanisms -- Prediction of Folding and Unfolding Rates of Proteins with Simple Models -- Predicting and Simulating Mutational Effects on Protein Folding Kinetics -- Localization of Energetic Frustration in Proteins -- Modeling the Structure, Dynamics, and Transformations of Proteins with the UNRES Force Field.
520			_aThis volume provides comprehensive protocols on experimental and computational methods that are used to study probe protein folding reactions and mechanisms. Chapters divided into five parts detail protein engineering, protein chemistry, experimental approaches to investigate the thermodynamics and kinetics of protein folding transitions, probe protein folding at the single molecule, analysis and interpretation of computer simulations, procedures and tools for the prediction of protein folding properties. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Protein Folding: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
988			_aSpringer_Protocols_2022
650		7	_2embne _9139861 _aProteínas
776	0	8	_iPrinted edition: _z9781071617151
776	0	8	_iPrinted edition: _z9781071617175
776	0	8	_iPrinted edition: _z9781071617182
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1716-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI