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020 _a9781071625255
024 7 _a10.1007/978-1-0716-2525-5
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH631
_b2022 EB
245 0 0 _aSingle Cell 'Omics of Neuronal Cells
_cedited by Jonathan V. Sweedler, James Eberwine, Scott E. Fraser
250 _a1st edition 2022
264 1 _aNew York, NY
_bSpringer International Publising
_c2022
300 _a1 recurso en línea (XIV, 294 páginas)
_b58 ilustraciones, 56 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aNeuromethods
_x1940-6045
_v184
505 0 _aSinge Cell aRNA-Seq for Transcriptomics and Cellular Phenotyping -- Single Cell Genomic and Epigenomic DNA Analyses -- Single-Cell Proteome Profiling of Neuronal Cells -- Joint Analysis of Transcriptome and Proteome Measurements in Single Cells with TotalVI -- Single-Cell Mass Spectrometry of Metabolites and Proteins for Systems and Functional Biology -- High-Throughout Image-Guided Microprobe Mass Spectrometric Analysis of Single Cells -- Single-Cell Resolution Immunoblotting -- Quantifying Enzyme Activity and Gene Expression within Single-Cells using a Multiplexed Capillary Electrophoresis Platform -- Quantitative Chemical Imaging at the Cell Level-SIMS, Fluorescence, and Correlative Techniques -- Electrophysiology and Single Cells -- Genetically Encoded Biosensors Reveal Spatiotemporal Dynamics and Cellular Heterogeneity of Neuronal Cells.
520 _aThis volume discusses the latest techniques used in the diverse fields of single cell 'omics and covers topics such as quantifying the single cell transcriptome; isolation of cells in nanoliter volumes for single cell proteomics measurements by nano-LC-MS/MS; and single cell protein characterization by immunoblotting. A wide range of methodologies are highlighted, ranging from high-yield chemical amplification to mass spectrometry and nanotechnology for the analysis of the chemical constituents of cells. In the Neuromethods series style, chapters include both in-depth overviews, as well as detailed protocols that provide the key advice from specialists you need to get successful results in your laboratory. Cutting-edge and comprehensive, Single Cell 'Omics of Neuronal Cells is a valuable resource for experienced and novice researchers interested in learning more about this field and its future developments.
988 _aSpringer_Protocols_2022
650 7 _2embne
_9165668
_aFisiología celular
776 0 8 _iPrinted edition:
_z9781071625248
776 0 8 _iPrinted edition:
_z9781071625262
776 0 8 _iPrinted edition:
_z9781071625279
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2525-5
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b07/2023
_dz
_eb
_zSI