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008			220518s2022 xxua o |||| 0|eng d
020			_a9781071622339
024	7		_a10.1007/978-1-0716-2233-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH443 _b2022 EB
245	0	0	_aRecombineering : _bMethods and Protocols _cedited by Christopher R. Reisch
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 264 páginas) _b57 ilustraciones, 50 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2479
505	0		_aScarless ssDNA Recombineering of Phage in the Lysogenic State -- Lambda red Recombineering of Bacteriophage in the Lysogenic State -- Recombineering Mediated Genome Editing in Burkholderiales Strains -- High-Efficiency Multi-Site Genomic Editing (HEMSE) Made Easy -- Creation of Markerless Genome Modifications in a Non-Model Bacterium by Fluorescence-Aided Recombineering -- Plant Gene Modification by BAC Recombineering -- Dup-In and DIRex: Techniques for Single-Step, Scar-Free Mutagenesis with Marker Recycling -- Genome Editing in Klebsiella pneumoniae using CRISPR/Cas9 Technology -- Generating Single Nucleotide Point Mutations in E. coli with the No-SCAR System -- Recombineering in Staphylococcus aureus -- Genome Editing Methods for Bacillus subtilis -- Highly Efficient Genome Editing in Clostridium difficile using the CRISPR-Cpf1 System -- Genome Editing of Corynebacterium glutamicum using CRISPR-Cpf1 System -- CRISPR/Cas-Mediated Genome Editing of Streptomyces -- Cas9 Nickase-Based Genome Editing in Clostridium cellulolyticum -- Genome Editing with Cas9 in Lactobacilli.
520			_aThis volume explores a collection of methods that studies genome editing across a variety of bacteria, phages, and plants. Chapters in this book cover topics such as scarless DNA recombineering of phage in the lysogenic state; HEMSE; Dup-In and DIRex; recombineering in Staphylococcus aureus; and genome editing with Cas9 in lactobacilli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Recombineering: Methods and Protocols is a valuable resource for any researchers interested in learning more about this developing field. .
988			_aSpringer_Protocols_2022
650		7	_2embne _9140562 _aGenética humana
776	0	8	_iPrinted edition: _z9781071622322
776	0	8	_iPrinted edition: _z9781071622346
776	0	8	_iPrinted edition: _z9781071622353
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2233-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eb _zSI