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008			220518s2022 xxua o |||| 0|eng d
020			_a9781071621769
024	7		_a10.1007/978-1-0716-2176-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP519.7 _b2022 EB
245	0	0	_aAffinity Chromatography : _bMethods and Protocols _cedited by B. Vijayalakshmi Ayyar, Sushrut Arora
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (X, 278 páginas) _b52 ilustraciones, 42 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2466
505	0		_aAntibody Purification Using Affinity Chromatography -- TIM4-affinity Methods Targeting Phosphatidylserine for Isolation or Detection of Extracellular Vesicles -- Application of Affitins for Affinity Purification of Proteins -- Identification of Clusterin as a major ABri- and ADan-binding protein using affinity Chromatography -- Application of a Novel CL7/Im7 Affinity System in Purification of Complex and Pharmaceutical Proteins -- Positive and Negative Affinity Chromatography to Purify Norovirus P-Domain Protein -- Robotic Affinity Purification of Soluble and Insoluble Recombinant Glutathione-S-Transferase Fusion Proteins -- Application of Immunoaffinity-Mass Spectrometry (IA-MS) for Protein Biomarker Quantification -- Cellular Membrane Affinity Chromatography for the Identification of Bioactive Molecules from Complex Natural Mixtures -- Rapid Affinity Purification of Tagged Plant Mitochondria (Mito-AP) -- Arginine-affinity Chromatography for Nucleic Acids (DNA and RNA) Isolation -- Purification and Analysis of Nucleotides and Nucleosides from Plants -- Ligand Selection for Affinity Chromatography Using Phage Display -- Selection and Application of Aptamer Affinity for Protein Purification -- Entrapment of Proteins within Columns for High-Performance Affinity Chromatography -- Preparation of Affinity Chromatography Monolith in Miniaturized Format and Application for Protein Separation -- Ligand Immobilization Methods for Affinity Chromatography -- Preparation of Molecularly Imprinted poly(N-isopropylacrylamide) Thermosensitive Based Cryogels -- Preparation of Staphylococcal Protein a Imprinted Supermacroporous Cryogel Beads.
520			_aThis volume covers various aspects of affinity chromatography along with examples of its applications. Chapters guide readers through methodologies to purify a diverse array of molecular targets such as antibodies, extracellular vesicles, recombinant proteins, biomarkers, metabolites, plant organelles, nucleic acids, ligand identification and protocols on building affinity matrix. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Affinity Chromatography: Methods and Protocols aims to be an invaluable resource to anyone employing affinity chromatography-based methodologies.
988			_aSpringer_Protocols_2022
650		7	_2embne _9149316 _aBioquímica clínica
650		7	_2embne _9683072 _aCromatografía
776	0	8	_iPrinted edition: _z9781071621752
776	0	8	_iPrinted edition: _z9781071621776
776	0	8	_iPrinted edition: _z9781071621783
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2176-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI