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008			220518s2022 xxua o |||| 0|eng d
020			_a9781071620793
024	7		_a10.1007/978-1-0716-2079-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK725 _b2022 EB
245	0	0	_aPlant Proteases and Plant Cell Death : _bMethods and Protocols _cedited by Marina Klemenčič, Simon Stael, Pitter F. Huesgen
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 299 páginas) _b58 ilustraciones, 53 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2447
505	0		_aExpression and purification of the type I metacaspase from a cryptophyte Guillardia theta, GtMCA-I -- Expression and purification of the type II metacaspase from a unicellular green alga Chlamydomonas reinhardtii -- Expression in Escherichia coli, refolding, and purification of plant aspartic proteases -- Production of functional plant legumain proteases using the Leishmania tarentolae expression system -- Purification of His-tagged proteases from the apoplast of agroinfiltrated N. benthamiana -- Identification of cognate protease/substrate pairs by use of class-specific inhibitors -- Peptide backbone modifications for the assessment of cleavage site relevance in precursors of signaling peptides -- Detection of apoplastic protease inhibitors using convolution activity based proteome profiling -- Cysteine reactivity profiling to unveil redox regulation in phytopathogens -- Determination of caspase-like activities in roots by the use of fluorogenic substrates -- Detection of damage-activated metacaspase activity by Western blot in plants -- Sensitive plant N-terminome profiling with HUNTER -- Profiling sequence specificity of proteolytic activities using proteome-derived peptide libraries -- Monitoring Cell Death via Ion leakage and PAM Fluorometry -- Measuring and perturbing ferroptosis in plants -- Detection and quantification of the hypersensitive response cell death in Arabidopsis thaliana -- Monitoring of ATG4 protease activity during autophagy in the model microalga Chlamydomonas reinhardtii -- Oxidative stress in roots - detection of lipid peroxidation and total antioxidative capacity -- Imaging of potassium and calcium distribution in plant tissues and cells to monitor stress response and programmed cell death -- Monitoring Pseudomonas syringae growth in agroinfiltrated leaves: the 'agromonas' assay -- Analysis of virus spread around the cell death zone at spatiotemporal resolution using confocal microscopy -- A user guide to validation, annotation and evaluation of N-terminome datasets with MANTI -- Exploring posttranslational modifications with the Plant PTM Viewer. .
520			_aThis volume presents current methods to detect and measure the activity of proteolytic enzymes in organisms ranging from unicellular algae to flowering plants. Chapters detail in vitro production, characterization of plant proteases, tools for in vivo modifications, proteomic approaches for identification of substrates, inhibitors and interacting partners, and the function of plant proteases in plant programmed cell death. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Proteases: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. .
988			_aSpringer_Protocols_2022
650		7	_2embne _9146302 _aCélulas vegetales
650		7	_2embne _9160237 _aMuerte celular
776	0	8	_iPrinted edition: _z9781071620786
776	0	8	_iPrinted edition: _z9781071620809
776	0	8	_iPrinted edition: _z9781071620816
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2079-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI