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008			220516s2022 xxua o |||| 0|eng d
020			_a9781071623336
024	7		_a10.1007/978-1-0716-2333-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aSF971 _b2022 EB
245	0	0	_aAfrican Swine Fever Virus : _bMethods and Protocols _cedited by Christopher L. Netherton
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XII, 218 páginas) _b44 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2503
505	0		_aWorking safely with African swine fever virus -- Necropsy procedures and evaluation of macroscopic lesions of pigs infected with African swine fever virus -- Preparation of immunofluorescently labelled tissue sections for imaging at low and high magnifications in the confocal microscope -- Primary macrophage culture from porcine blood and lungs -- Isolation of porcine bone marrow cells and generation of recombinant African swine fever viruses -- Laboratory diagnosis and quantification of African swine fever virus using real-time polymerase chain reaction -- Quantification of ASFV DNA and RNA in Ornithodoros soft ticks -- Genotyping of African swine fever virus -- African swine fever virus (ASFV) indirect ELISA test based on the use of the soluble cytoplasmic semi purified antigen (ASFV CP-Ag) -- Indirect immunoperoxidase test (IPT) for detection of antibodies against African swine fever virus (ASFV) on African green monkey cell lines (Vero, MS) -- African Swine Fever Virus Haemadosrption Inhibition Assay -- ELISpot assay for the detection of ASFV-specific interferon-gamma (IFN-γ) producing cells -- Purification of African swine fever virus -- African swine fever virus plaque assay and disinfectant testing -- Bioorthogonal labelling of African swine fever virus infected cells -- Whole genome sequencing of African swine fever virus.
520			_aThis volume provides a compilation of methods that will aid researchers studying virology, immunology, and vaccinology of this devastating disease of swine. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, African Swine Fever Virus: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field. .
988			_aSpringer_Protocols_2022
650		7	_2embne _9139527 _aCerdos _xEnfermedades
776	0	8	_iPrinted edition: _z9781071623329
776	0	8	_iPrinted edition: _z9781071623343
776	0	8	_iPrinted edition: _z9781071623350
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2333-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI