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008			220314s2022 xxua o |||| 0|eng d
020			_a9781071622452
024	7		_a10.1007/978-1-0716-2245-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP620 _b2022 EB
245	0	0	_acAMP Signaling : _bMethods and Protocols _cedited by Manuela Zaccolo
250			_a2nd edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XII, 369 páginas) _b85 ilustraciones, 75 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2483
505	0		_aReal-Time Measurements of Intracellular cAMP Gradients using FRET-Based cAMP Nanorulers -- Assaying Protein Kinase A Activity using a FRET-Based Sensor Purified from Mammalian Cells -- MultiFRET: A Detailed Protocol for High-Throughput Multiplexed Ratiometric FRET -- Photoactivated Adenylyl Cyclases as Optogenetic Modulators of Neuronal Activity -- Imaging the cAMP Signaling Microdomain of the Primary Cilium using Targeted FRET-Based Biosensors -- Methods to Assess Phosphodiesterase and/or Adenylyl Cyclase Activity via Heterologous Expression in Fission Yeast -- Time-Domain Fluorescence Lifetime Imaging of cAMP Levels with EPAC-Based FRET Sensors -- Disruptors of AKAP-Dependent Protein-Protein Interactions -- Micro-2D Cell Culture for cAMP Measurements using FRET Reporters in Human iPSC-Derived Cardiomyocytes -- Automated Image Analysis of FRET Signals for Subcellular cAMP Quantification -- In Vivo cAMP Dynamics in Drosophila Larval Neurons -- Live Cell Imaging of Cyclic Nucleotides in Human Cardiomyocytes -- Optogenetic Control of Heart Rhythm: Lightly Guiding the Cardiac Pace -- Live Imaging of cAMP Signaling in D. discoideum Based on a Bright Bioluminescent Protein, Nano-Lantern -- Generation of Transgenic Mice Expressing Cytosolic and Targeted FRET Biosensors for cAMP and cGMP -- How to Make the CUTiest Sensor in 3 Simple Steps for Computational Pedestrians -- Ion-Channel Based Reporters for cAMP Detection -- Quantitative Phosphoproteomics to Study cAMP Signalling -- Biochemical Analysis of AKAP-Anchored PKA Signaling Complexes -- Fluorescent Translocation Reporters for Sub-Plasma Membrane cAMP Imaging -- A Live-Cell Imaging Assay for Nuclear Entry of cAMP-Dependent Protein Kinase Catalytic Subunits Stimulated by Endogenous GPCR Activation -- Measuring Spatiotemporal cAMP Dynamics within an Endogenous Signaling Compartment using FluoSTEP-ICUE.
520			_aThis volume discusses the latest techniques used by researchers to measure cAMP activity at the cell population, whole cell, and subcellular level. The techniques covered in the book address questions related to cAMP compartmentalization, which look at relevant protein-protein interactions that increase the spatial and temporal resolution of cAMP signals detection, and that can help in the integration of the increasingly complex information that is becoming available in this field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, cAMP Signaling: Methods and Protocols, Second Edition is a valuable resource for scientists and researchers who are interested in learning more about this important and developing field. Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
988			_aSpringer_Protocols_2022
650		7	_2embne _9140525 _aÁcidos nucleicos
776	0	8	_iPrinted edition: _z9781071622445
776	0	8	_iPrinted edition: _z9781071622469
776	0	8	_iPrinted edition: _z9781071622476
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2245-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI