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008			220308s2022 xxua o |||| 0|eng d
020			_a9781071621646
024	7		_a10.1007/978-1-0716-2164-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK725 _b2022 EB
245	0	0	_aProtoplast Technology : _bMethods and Protocols _cedited by Kan Wang, Feng Zhang
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XII, 263 páginas) _b43 ilustraciones, 40 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2464
505	0		_aPhyscomitrium patens Protoplasting and Protoplast Transfection -- Protoplast Isolation and Transfection in the Single-Cell C4 Species Bienertia sinuspersici -- Protoplast for Gene Functional Analysis in Arabidopsis -- Application of Protoplast Regeneration to CRISPR/Cas9 Mutagenesis in Nicotiana tabacum -- CRISPR DNA- and RNP-Mediated Genome Editing via Nicotiana benthamiana Protoplast Transformation and Regeneration -- Protoplast Isolation and Transfection in Rice -- Protoplast Isolation and Transfection in Maize -- Isolation and Transfection of Maize Endosperm Protoplasts -- Protoplast Isolation from Undifferentiated Maize Seedling Shoot Tissue -- Protoplast Isolation and Transfection in Wheat -- Protoplast Isolation, Transformation, and Regeneration for Forage and Turf Grasses -- Efficient Protoplast Isolation and DNA Transfection for Winter Oilseed Crops, Pennycress (Thlaspi arvense) and Camelina (Camelina sativa) -- Protoplast Isolation, Transfection, and Gene Editing for Soybean (Glycine max) -- Protoplast Isolation and Transformation in Oil Palm -- Protoplast Preparation and Fluorescence-Activated Cell Sorting for the Evaluation of Targeted Mutagenesis in Plant Cells -- Characterization of Programmable Transcription Activators in the Model Monocot Setaria viridis via Protoplast Transfection -- High-Throughput Transfection and Analysis of Soybean (Glycine max) Protoplasts.
520			_aThis detailed volume collects protocols from scientists who are actively engaged in developing or using protoplast technology. The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and regeneration, as well as examples of how to use protoplasts for genome editing and gene function analysis in a number of major crop or model plant species. It continues by exploring protoplast automation, large scale functional genomics, and synthetic biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protoplast Technology: Methods and Protocols aims to inspire the new generation of researchers to further improve their protocols and apply this technology to accelerate the field of plant genomic study.
988			_aSpringer_Protocols_2022
650		7	_2embne _9146302 _aCélulas vegetales
776	0	8	_iPrinted edition: _z9781071621639
776	0	8	_iPrinted edition: _z9781071621653
776	0	8	_iPrinted edition: _z9781071621660
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2164-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI