000			04076nam a2200409 i 4500
999			_c396531 _d396531
001			396531
003			ES-MaUEC
005			20230713102206.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			211123s2022 xxua o |||| 0|eng d
020			_a9781071618264
024	7		_a10.1007/978-1-0716-1826-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP601 _b2022 EB
245	0	0	_aEnzyme Engineering : _bMethods and Protocols _cedited by Francesca Magnani, Chiara Marabelli, Francesca Paradisi
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 360 páginas) _b84 ilustraciones, 69 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2397
505	0		_aPreparation of Soil Metagenome Libraries and Screening for Gene Specific Amplicons -- Ultrahigh-Throughput Screening of Metagenomic Libraries Using Droplet Microfluidics -- Synthetic DNA Libraries for Protein Engineering toward Process Improvement in Drug Synthesis -- In Silico Prediction Methods for Site-Saturation Mutagenesis -- Recombination of Compatible Substitutions by 2GenReP and InSiReP -- Using the Evolutionary History of Proteins to Engineer Insertion-Deletion Mutants from Robust, Ancestral Templates Using Graphical Representation of Ancestral Sequence Predictions (GRASP) -- Resurrecting Enzymes by Ancestral Sequence Reconstruction -- Expression and In Vivo Loading of De Novo Proteins with Tetrapyrrole Cofactors -- Rational-Design Engineering to Improve Enzyme Thermostability -- Using Molecular Simulation to Guide Protein Engineering for Biocatalysis in Organic Solvents -- In Silico Engineering of Enzyme Access Tunnels -- Quantum-Mechanical/Molecular-Mechanical (QM/MM) Simulations for Understanding Enzyme Dynamics -- Computational Enzyme Design at Zymvol -- Advanced Enzyme Immobilization Technologies: An Eco-Friendly Support, a Polymer-Stabilizing Immobilization Strategy, and an Improved Cofactor Co-Immobilization Technique -- Chemical Reaction Engineering to Understand Applied Kinetics in Free Enzyme Homogeneous Reactors -- Enzymatic Oxidative Cascade for Oxofunctionalization of Fatty Acids in One-Pot -- CRISPR-Cas9 Editing of the Synthesis of Biodegradable Polyesters Polyhydroxyalkanaotes (PHA) in Pseudomonas putida KT2440.
520			_aThis book provides comprehensive methods and protocols about enzyme design. The chapters are grouped by main topic, starting with methodologies describing library preparation and screening, state of the art techniques in directed evolution and rational design, followed by examples of immobilization of enzymes on sustainable polymers, as well as biocatalytic conversions mediated by homogenous enzymatic preparations or whole cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, Enzyme Engineering: Methods and Protocols is an ideal guide for both the novice and the veteran researcher interested in biocatalysis. Chapter 13 is available open access under a CC BY 4.0 license.
988			_aSpringer_Protocols_2022
650		7	_2embne _9138640 _aEnzimas
650		7	_2embne _9143820 _aIngeniería biomédica
776	0	8	_iPrinted edition: _z9781071618257
776	0	8	_iPrinted edition: _z9781071618271
776	0	8	_iPrinted edition: _z9781071618288
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1826-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb