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008			211121s2022 xxua o |||| 0|eng d
020			_a9781071617366
024	7		_a10.1007/978-1-0716-1736-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR185.8 .T2 _b2022 EB
245	0	0	_aT-Follicular Helper Cells : _bMethods and Protocols _cedited by Luis Graca
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 294 páginas) _b69 ilustraciones, 51 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2380
505	0		_aComplete Visualization of T Follicular Helper Cells in Germinal Centers -- In vivo imaging of Tfh cells -- Circulating T Follicular Helper Subsets in Human Blood -- Identification of Human Circulating and Tissue T Follicular Regulatory (Tfr) Cells by Flow Cytometry -- Assessment of circulating Tfh and Tfr by mass cytometry -- Identification of T peripheral helper (Tph) cells -- Identification of non-classical follicular T cells -- Identification of follicular T cells in the gut -- Intravital imaging of Tfh dynamics -- A method to analyze models of the dynamics of GCs -- Identification of Antigen Presenting Cell subsets supporting human Tfh differentiation Lamine Alaoui, Melanie Durand, and Elodie Segura -- The use of peptide-MHCII tetramers to identify antigen-specific T follicular helper and T follicular regulatory cells -- Assessment of the TCR repertoire of human circulating T follicular helper cells -- Assessing in vivo T cell-dependent antigen-specific antibody responses -- Tfh-mediated and Tfr-suppressed antigen driven IgG and IgE assays -- Assessment of the impact of cytokines on TFH, TREG, and TFR cell populations after influenza infection -- Evaluation of human circulating T follicular helper cells in influenza- and SARS-CoV-2-specific B cell immunity -- Detection of T follicular helper cells and T follicular regulatory cells in experimental Sjögren's syndrome -- Identification of Human T Follicular Cells in Ectopic Lymphoid Structures -- Understanding T Follicular helper cells in asthma through murine models of allergic airway disease -- Therapeutic modulation of T follicular helper cells by low-dose IL-2 treatment -- 3D tissue explant and single-cell culture systems for ex vivo drug testing on Tfh cells derived from human adenoid tonsils.
520			_aThe volume provides a comprehensive overview of methods that have been developed for the study of Tfh and Tfr biology in mice, and in different human diseases. The chapters detail methods to investigate the function of Tfh cells in vivo and in vitro, including the use of mathematical models to study GC dynamics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T-Follicular Helper Cells: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.
988			_aSpringer_Protocols_2022
650		7	_2embne _9144344 _aLinfocitos T
650		7	_2embne _9165668 _aFisiología celular
776	0	8	_iPrinted edition: _z9781071617359
776	0	8	_iPrinted edition: _z9781071617373
776	0	8	_iPrinted edition: _z9781071617380
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1736-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI