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020 _a9781071618226
024 7 _a10.1007/978-1-0716-1822-6
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQK881
_b2022 EB
245 0 0 _aPlant Metabolic Engineering :
_bMethods and Protocols
_cedited by Vladimir Shulaev
250 _a1st edition 2022
264 1 _aNew York, NY
_bSpringer International Publising
_c2022
300 _a1 recurso en línea (X, 244 páginas)
_b69 ilustraciones, 55 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2396
505 0 _aMethods for the Development of Recombinant Microorganisms for the Production of Natural Products -- Sustainable Technological Methods for the Extraction of Phytochemicals from Citrus Byproducts -- Reconstitution of Metabolic Pathway in Nicotiana benthamiana -- A Protocol for Phylogenetic Reconstruction -- RNA-Seq Data Analysis Pipeline for Plants: Transcriptome Assembly, Alignment, and Differential Expression Analysis -- A Protocol for Horizontally Acquired Metabolic Gene Detection in Algae -- Global Comparative Label-Free Yeast Proteome Analysis by LC-MS/MS After High-pH Reversed-Phase Peptide Fractionation Using Solid-Phase Extraction Cartridges -- Gas Chromatography Coupled to Atmospheric Pressure Chemical Ionization High Resolution Mass Spectrometry for Metabolite Fingerprinting of Grape (Vitis vinifera L.) Berry -- GC-MS/MS Profiling of Plant Metabolites -- Analysis of Grape Volatiles using Atmospheric Pressure Ionization Gas Chromatography Mass Spectrometry-Based Metabolomics -- A High-Throughput HILIC-MS-Based Metabolomic Assay for the Analysis of Polar Metabolites -- Macrolipidomic Profiling of Vegetable Oils: The Analysis of Sunflower Oils with Different Oleic Acid Content -- Non-Targeted Lipidomics using a Robust and Reproducible Lipid Separation using UPLC with Charged Surface Hybrid Technology and High Resolution Mass Spectrometry -- Comprehensive Analysis of Plant Lipids using Sub-2µm Particle CO2-Based Chromatography Coupled to Mass Spectrometry -- Bioinformatics in Lipidomics: Automating Large-Scale LC-MS-Based Untargeted Lipidomics Profiling with SimLipid Software -- A Protocol for Prion Discovery in Plants -- Structural Determination of Uridine Diphosphate Glycosyltransferases using X-Ray Crystallography.
520 _aThis volume looks at the latest techniques used by researchers to study various aspects of plant metabolic engineering. The chapters in this book cover topics such as bioinformatics tools used to discover new genes and pathways; heterologous expression of biosynthetic genes in plant and microbial systems; and omics technologies, such as transcriptomics, proteomics, metabolomics, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Metabolic Engineering: Methods and Protocols is a valuable resource for biologists, chemists, biotechnologists, students, and broad cohorts of researchers who works in the fields of plant metabolism and metabolic engineering. .
988 _aSpringer_Protocols_2022
650 7 _2embne
_9168920
_aPlantas
_xMetabolismo
650 7 _2embne
_9485040
_aIngeniería bioquímica
776 0 8 _iPrinted edition:
_z9781071618219
776 0 8 _iPrinted edition:
_z9781071618233
776 0 8 _iPrinted edition:
_z9781071618240
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1822-6
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b07/2023
_dz
_eb
_zSI