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008			211113s2022 xxua o |||| 0|eng d
020			_a9781071617991
024	7		_a10.1007/978-1-0716-1799-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP601 _b2022 EB
245	0	0	_aPCR Primer Design _cedited by Chhandak Basu
250			_a3rd edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIII, 276 páginas) _b90 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2392
505	0		_aThe significance of PCR primer design in genetic diversity studies; exemplified by recent research into the genetic structure of marine species -- Enhancing cohort PASA efficiency from lessons assimilated by mutant genotyping in C. elegans -- Design of oligonucleotides for allele-specific amplification based on PCR and isothermal techniques -- Detection of rubella virus by tri-primer RT-PCR assay and genotyping by fragment RT-PCR -- Design of mismatch primers to identify and differentiate closely related (sub)species - application to the authentication of meat products -- Primer design for the analysis of closely related species - application of non-coding mtDNA and cpDNA sequences -- Designing PCR primers for the amplification-refractory mutation system -- Validation of circular RNAs by PCR -- Primer Designing for Amplifying an AT-Rich Promoter from Arabidopsis thaliana -- PLASmid TAXonomic PCR (PlasTax-PCR), a multiplex relaxase MOB typing to assort plasmids into taxonomic units -- Multiplex PCR Design for Scalable Resequencing -- Identification of gene copy number in the transgenic plants by quantitative polymerase chain reaction (qPCR) -- qPrimerDB: A powerful and user-friendly database for qPCR primer design -- PCR primer design for the rapidly evolving SARS-CoV-2 genome -- Universal primers for detection of novel plant capsid-less viruses: Papaya umbra-like viruses as example -- A guide to using FASTPCR software for PCR, in silico PCR, and oligonucleotide analysis -- Pyrosequencing Primer Design for Forensic Biology Applications.-Phosphate‌ ‌methylated‌ ‌oligonucleotides‌ ‌as‌ ‌a‌ ‌novel‌ ‌primer‌ ‌for‌ ‌PCR‌ ‌and‌ ‌RT-PCR‌. .
520			_aThis third edition provides new and updated chapters on design PCR primers for successful DNA amplification. Chapters are divided into seven parts, including primer design strategies for quantitative PCR, genotyping, multiplex PCR, in silico PCR primer design, and primer design to identify plant and animal viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, PCR Primer Design, Third Edition aims to be useful for various fields of molecular biology, including biotechnology, molecular genetics, and recombinant DNA technology.
988			_aSpringer_Protocols_2022
650		7	_2embne _9138640 _aEnzimas
776	0	8	_iPrinted edition: _z9781071617984
776	0	8	_iPrinted edition: _z9781071618004
776	0	8	_iPrinted edition: _z9781071618011
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1799-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI