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008			211111s2022 xxua o |||| 0|eng d
020			_a9781071617717
024	7		_a10.1007/978-1-0716-1771-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2022 EB
245	0	0	_aSingle-Cell Protein Analysis : _bMethods and Protocols _cedited by Aik T. Ooi
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (X, 311 páginas) _b95 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2386
505	0		_aImmunochemistry Analysis Using Chromogenic Substrates on Tissue Sections -- Indirect Immunofluorescence of Tissue Sections -- Flow Cytometry for Beginners: Hints and Tips for Approaching the Very First Single-Cell Technique -- High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry -- Detection of Cytokine Secreting Cells by Enzyme-Linked Immunospot (ELISpot) -- Detection and Enumeration of Cytokine Secreting Cells by FluoroSpot -- Single Cell Protein Profiling by Microdroplet Barcoding and Next Generation Sequencing -- Single Cell Proteomics Using Multiplexed Isobaric Labeling for Mass Spectrometric Analysis -- Customizing Maxpar Direct Immune Profiling Assay with Additional Surface Marker and Intracellular Cytokine Staining Workflows for Expanded Mass Cytometry Panels -- High-Dimensional Tissue Profiling by Multiplexed Ion Beam Imaging -- Ultra-Sensitive Quantification of Protein and mRNA in Single Mammalian Cells with Digital PLA -- High-Throughput Multimodal Single-Cell Targeted DNA and Surface Protein Analysis Using the Mission Bio Tapestri Platform -- Simultaneous Analysis of Single-Cell Transcriptomes and Cell Surface Protein Expression of Human Hematopoietic Stem Cells and Progenitors Using the 10x Genomics Platform -- Generation of Centered Log-Ratio Normalized Antibody-Derived Tag Counts from Large Single-Cell Sequencing Datasets -- Simultaneous Quantification of Single-Cell Proteomes and Transcriptomes in Integrated Fluidic Circuits -- Combined Measurement of RNA and Protein Expression on a Single-Cell Level -- Simultaneous DNA, RNA, and Protein Analysis from Single Cells Using a High-Throughput Microfluidic Workflow for Resolution of Genotype-to-Phenotype Modalities.
520			_aThis detailed volume serves as a collection of methods for single-cell protein analysis, created by combining different protocols, taking advantage of new emerging technologies, and improving upon conventional methods to guide researchers aiming to perform protein analysis in single cells. Ranging from simple to complex, conventional to the most current technologies, these chapters offer readers the ability to choose the best suited methodologies for them, based on the sample type and the available technologies or equipment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Single-Cell Protein Analysis: Methods and Protocols aims to help researchers utilizing single-cell protein analysis in their studies as well to inspire the development of the next generation of improved protein analysis methods in single cells.
988			_aSpringer_Protocols_2022
650		7	_2embne _9242917 _aProteínas
650		7	_2embne _9430647 _aProteómica
776	0	8	_iPrinted edition: _z9781071617700
776	0	8	_iPrinted edition: _z9781071617724
776	0	8	_iPrinted edition: _z9781071617731
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1771-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI