000			04167nam a2200409 i 4500
999			_c396520 _d396520
001			396520
003			ES-MaUEC
005			20230720134037.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			211028s2022 xxua o |||| 0|eng d
020			_a9781071618790
024	7		_a10.1007/978-1-0716-1879-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR398 _b2022 EB
245	0	0	_aDengue Virus : _bMethods and Protocols _cedited by Ronaldo Mohana-Borges
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 295 páginas) _b65 ilustraciones, 56 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2409
505	0		_aVirus Propagation and Plaque Assay for Dengue Virus -- Transmission Electron Microscopy Studies of Dengue Viruses -- Production of Properly Folded NS1 Protein in Bacterial Cells -- Expression and Purification of Dengue NS1 Protein in Sf9-Baculovirus System -- Purification of Dengue and Zika Virus Non-Structural Protein 5 for Crystallization and Screening of Antivirals -- Obtention of Dengue Virus Membrane Proteins and Role for Virus Assembly -- Construction of Genomic and Sub-Genomic Dengue Infectious Replicons -- Dengue Virus Capsid-Protein Dynamics in Live Infected Cells Studied by Pair Correlation Analysis -- Yeast Two-Hybrid System for Mapping Novel Dengue Protein Interactions -- Isolation and Identification of Dengue Virus Interactome with Human Plasma Proteins by Affinity Purification-Mass Spectrometry -- Molecular Diagnosis Of Dengue -- Serological Diagnosis of Dengue -- Measuring Transendothelial Electrical Resistance (TEER) for Dengue Infection Studies -- Evaluation of DENV-Induced Endothelial Cell Permeability by Measurements of Transendothelial Electrical Resistance (TEER) and Extravasation of Proteins and Virus -- In Vitro Cytokine Production by Dengue-Infected Human Monocyte-Derived Dendritic Cells -- In Vitro Generation of Human Antibody-Secreting Cells through the Stimulation of PBMCs with Dengue Virus Particles -- Isolation of Microvesicles from Plasma Samples Avoiding Lipoprotein Contamination -- Evaluating Dengue Virus Pathogenesis in Mice and Humans by Histological and Immunohistochemistry Approaches -- Humanized Mice for the Study of Dengue Disease Pathogenesis: Biological Assays.
520			_aThis detailed volume explores various aspects of the dengue virus and its four serotypes (DENV1-4). Beginning with a section on protocols to produce DENV and its proteins that are fundamental for many biophysical, biochemical, immunological, and cellular studies, the book continues by covering protocols to study the interactions between DENV and cellular proteins, DENV immunopathogenesis and diagnosis, as well as recent advances in animal models that can be used in studies of DENV immunopathogenesis and vaccine development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Dengue Virus: Methods and Protocols serves as an ideal guide for researchers attempting to further understand this critical threat to worldwide public health.
988			_aSpringer_Protocols_2022
650		7	_2embne _9677583 _aArbovirus
650		7	_2embne _9413118 _aEnfermedades víricas
776	0	8	_iPrinted edition: _z9781071618783
776	0	8	_iPrinted edition: _z9781071618806
776	0	8	_iPrinted edition: _z9781071618813
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1879-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI