000			05014nam a2200409 i 4500
999			_c396519 _d396519
001			396519
003			ES-MaUEC
005			20230710120635.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			211028s2022 xxua o |||| 0|eng d
020			_a9781071617205
024	7		_a10.1007/978-1-0716-1720-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH447 _b2022 EB
245	0	0	_aEssential Genes and Genomes : _bMethods and Protocols _cedited by Ren Zhang
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XII, 434 páginas) _b68 ilustraciones, 50 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2377
505	0		_aA Method to Map Gene Essentiality of Human Pluripotent Stem Cells by Genome-Scale CRISPR Screens with Inducible Cas9 -- CRISPR/Cas9 Screening to Identify Conditionally Essential Genes in Human Cell Lines -- Target Identification of Small Molecules Using Large-Scale CRISPR-Cas Mutagenesis Scanning of Essential Genes -- Design and Generation of a CRISPR Interference System for Genetic Repression and Essential Gene Analysis in the Fungal Pathogen Candida albicans -- Identification of Essential Genes Using Sequential CRISPR and siRNA Screens -- A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell Lines -- New Method for Genome-Scale Functional Genomics Study in Bacteria with Superior Performance: CRISPR Interference Screen -- A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in Cyanobacteria -- A Method of Transposon Insertion Sequencing in Comprehensively Identifying Vibrio vulnificus Genes Required for Growth in Human Serum -- The Use of Tn-Seq and the FiTnEss Analysis to Define the Core Essential Genome of Pseudomonas aeruginosa -- Analysis of Escherichia coli K1 Virulence Genes by Transposon-Directed Sequencing -- Using Genome Scale Mutant Libraries to Identify Essential Genes -- Identification and Analysis of Essential Genes in Streptococcus mutans with Transposon Sequencing -- The Use of TnSeq to Identify Essential Alphaproteobacterial Genes Reveals Operational Variability in Conserved Developmental and Cell Cycle Systems -- A Proposed Framework to Identify Dispensable and Essential Functions in Bifidobacteria: Case Study of Bifidobacterium breve UCC2003 as a Prototype of Its Genus -- The Application of Transposon Insertion Sequencing in Identifying Essential Genes in B. fragilis -- Repressible Promoter System to Study Essential Genes in Mycobacteria -- A Single-Cell RNA-Seq-Based Approach for Genome-Wide Identification of Cell Essential Genes -- Identification of Essential Genes in Caenorhabditis elegans with Lethal Mutations Maintained by Genetic Balancers -- A Screening Method to Identify Essential Yeast Genes for Responses Towards Spermine -- An Effective Pre-Processing Method for High-Quality Pan-Genome Analysis of Bacillus subtilis and Escherichia coli -- Analysis of Gene Essentiality from TnSeq Data Using Transit -- Geptop 2.0: Accurately Select Essential Genes from the List of Protein-Coding Genes in Prokaryotic Genomes.
520			_aThis book provides state-of-the-art information on gene essentiality screenings in a wide variety of organisms, i.e. screening for protein-coding genes and other genomic elements that are required by an organism to survive under specific conditions. With a focus on the two techniques that have revolutionized the field, the collection begins with chapters employing CRISPR/Cas9-based approaches followed by Tn-seq-based approaches, but later chapters also delve into other techniques for exploring essential genes, such as bioinformatics methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Essential Genes and Genomes: Methods and Protocols is an ideal guide for researchers attempting to strip genetics down to its fundamentals.
988			_aSpringer_Protocols_2022
650		7	_2embne _9164326 _aGenómica
650		7	_2embne _9139993 _aGenes
776	0	8	_iPrinted edition: _z9781071617199
776	0	8	_iPrinted edition: _z9781071617212
776	0	8	_iPrinted edition: _z9781071617229
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1720-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI