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008			211025s2022 xxua o |||| 0|eng d
020			_a9781071618516
024	7		_a10.1007/978-1-0716-1851-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH431 _b2022 EB
245	0	0	_aPost-Transcriptional Gene Regulation _cedited by Erik Dassi
250			_a3rd edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIV, 411 páginas) _b71 ilustraciones, 64 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2404
505	0		_aIntroduction to Bioinformatics Resources for Post-Transcriptional Regulation of Gene Expression -- Predicting RNA Secondary Structure Using In Vitro and In Vivo Data -- RBPmap: A Tool for Mapping and Predicting the Binding Sites of RNA-Binding Proteins Considering the Motif Environment -- Analysis of mRNA Translation by Polysome Profiling -- Exploring Ribosome-Positioning on Translating Transcripts with Ribosome Profiling -- Identification of RNA Binding Partners of CRISPR-Cas Proteins in Prokaryotes Using RIP-Seq -- Rapidly Characterizing CRISPR-Cas13 Nucleases Using Cell-Free Transcription-Translation Systems -- Studying RNP Composition with RIP -- PAR-CLIP: A Method for Transcriptome-Wide Identification of RNA Binding Protein Interaction Sites -- A Pipeline for Analyzing eCLIP and iCLIP Data with htseq-clip and DEWSeq -- Identification of miRNAs Bound to an RNA of Interest by MicroRNA Capture Affinity Technology (miR-CATCH) -- Identifying the Protein Interactomes of Target RNAs Using HyPR-MS -- Visualization and Quantification of Subcellular RNA Localization Using Single Molecule RNA Fluorescence In Situ Hybridization -- Single-Molecule RNA Imaging Using Mango II Arrays -- Genome-Wide Identification of Polyadenylation Dynamics with TED-Seq -- In Vivo RNA Structure Probing with DMS-MaPseq -- Transcriptome-Wide Profiling of RNA Stability -- High-Throughput Quantitation of Yeast uORF Regulatory Impacts Using FACS-uORF -- m6A RNA Immunoprecipitation Followed by High-Throughput Sequencing to Map N6-Methyladenosine -- Detecting m6A with In Vitro DART-Seq -- Target-Specific Profiling of RNA m5C Methylation Level Using Amplicon Sequencing -- Transcriptome-Wide Identification of 2'-O-Methylation Sites with RibOxi-Seq.
520			_aThis volume presents the most recent advances in techniques for studying the post-transcriptional regulation of gene expression (PTR). With sections on bioinformatics approaches, expression profiling, the protein and RNA interactome, the mRNA lifecycle, and RNA modifications, the book guides molecular biologists toward harnessing the power of this new generation of techniques, while also introducing the data analysis skills that these high-throughput techniques require. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Post-Transcriptional Gene Regulation, Third Edition serves as a versatile resource for researchers studying post-transcriptional regulation by both introducing the most recent techniques and providing a comprehensive guide to their implementation. Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
988			_aSpringer_Protocols_2022
650		7	_2embne _9138044 _aGenética
650		7	_2embne _9160489 _aBioinformática
776	0	8	_iPrinted edition: _z9781071618509
776	0	8	_iPrinted edition: _z9781071618523
776	0	8	_iPrinted edition: _z9781071618530
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1851-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI