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008			211022s2022 xxua o |||| 0|eng d
020			_a9781071617953
024	7		_a10.1007/978-1-0716-1795-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aSB731 _b2022 EB
245	0	0	_aFusarium wilt : _bMethods and Protocols _cedited by Jeffrey Coleman
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 208 páginas) _b50 ilustraciones, 40 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2391
505	0		_a1. Pure culture and DNA sequence-based identification of Fusarium from symptomatic plants and diverse substrates -- Isolation of high-molecular weight (HMW) DNA from Fusarium oxysporum for long-read sequencing -- Karyotyping of Fusarium oxysporum by pulsed field gel electrophoresis and the germ tube burst method -- RNA-seq data analysis for differential expression -- Quantification and isolation of spontaneous colony growth variants -- Generation of a T-DNA insertion mutant library of Fusarium oxysporum using Agrobacterium tumefaciens -- Targeted gene disruption via CRISPR/Cas9 ribonucleoprotein complexes in Fusarium oxysporum -- CRISPR/Cas9 RNP-mediated gene fusion to assess protein quantification and subcellular localization in Fusarium oxysporum -- In vivo monitoring of cytosolic pH using the ratiometric pH sensor pHluorin -- Multi-pronged investigation of volatile compound-mediated interactions of Fusarium oxysporum with plants, fungi and bacteria -- Isolation of mitochondria from Fusarium species -- In planta gene expression analysis and colonization of Fusarium oxysporum -- Time-lapse imaging of root pathogenesis and fungal proliferation without physically disrupting roots -- High-throughput screening assays to identify plant natural products with antifungal properties against Fusarium oxysporum -- Screening and assessment of pisatin demethylase activity (PDA).-Detection and genotyping of Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), the Fusarium wilt pathogen of cotton.
520			_aThis volume provides a collection of molecular protocols detailing the most common and modern techniques on fusarium wilt. Chapters guide readers through methods on initial isolation, molecular-based identification, genome characterization, generation of mutants, and characterization of interactions with other organisms including host plants. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Fusarium wilt: Methods and Protocols aims to be a valuable resource for mycologists, plant pathologists, microbiologists, geneticists, and other scientists that have an interest in members of the Fusarium oxysporum species complex or closely related fungi. .
988			_aSpringer_Protocols_2022
650		7	_2embne _9139455 _aPatología vegetal
776	0	8	_iPrinted edition: _z9781071617946
776	0	8	_iPrinted edition: _z9781071617960
776	0	8	_iPrinted edition: _z9781071617977
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1795-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI