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008			211013s2022 xxua o |||| 0|eng d
020			_a9781071617793
024	7		_a10.1007/978-1-0716-1779-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC116 .M8 _b2022 EB
245	0	0	_aMycobacterium ulcerans : _bMethods and Protocols _cedited by Gerd Pluschke, Katharina Röltgen
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (X, 232 páginas) _b33 ilustraciones, 25 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2387
505	0		_aOverview: Mycobacterium ulcerans Disease (Buruli ulcer) -- Immunohistochemistry: A Method to Analyze M.ulcerans Infected Skin Tissue -- Primary Isolation of Mycobacterium ulcerans -- Investigation of Mycobacterium ulcerans Glycan Interactions Using Glycan Array and Surface Plasmon Resonance -- Purification and Characterization of Extracellular Vesicles from Mycobacterium ulcerans Culture -- DNA Extraction from Clinical Specimens for the Direct Detection of Mycobacterium ulcerans by Real-Time PCR -- Extraction of DNA from Environmental Samples for the Real-Time PCR Detection of Mycobacterium ulcerans -- Detection of Mycobacterium ulcerans DNA using Real-Time PCR -- Determining Viability of M. ulcerans by 16S rRNA RT Reverse Transcriptase Real-Time PCR -- Methods and Approaches for Buruli ulcer Surveillance in Africa: Lessons Learnt and Future Directions -- Overview: Mycolactone, The Macrolide Toxin of Mycobacterium ulcerans -- Conditions for Handling and Optimal Storage of Mycolactone -- Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples -- Detection of Mycolactone by Thin Layer Chromatography -- Competitive ELISA for the Detection and Quantification of Mycobacterium ulcerans Mycolactone -- Biochemical and Biological Assays of Mycolactone-Mediated Inhibition of Sec61 -- Overview: Development of Drugs against Mycobacterium ulcerans -- In Vitro Assessment of Drug Activity against Mycobacterium ulcerans -- Drug Efficacy Testing in the Mouse Footpad Model of Buruli ulcer -- Turbidity-Based MIC Assay and Characterization of Spontaneous Drug Resistant Mutants in Mycobacterium ulcerans -- Determination of Bioenergetic Parameters in Mycobacterium ulcerans.
520			_aThis volume explores the latest techniques used to study Mycobacterium ulcerans, and more specifically M. ulcerans disease (Buruli ulcer). The chapters in this book are organized into three parts and cover methods for the detection of M. ulcerans and the analysis of host-pathogen interaction; the quantification and characterization of mycolactone, the macrolide toxin of M. ulcerans; and drug development against M. ulcerans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mycobacterium ulcerans: Methods and Protocols is a valuable resource that helps scientists advance their research on Buruli ulcer, which is still an under-researched field in infection biology.
988			_aSpringer_Protocols_2022
650		7	_2embne _9669995 _aEnfermedades micobacterianas
776	0	8	_iPrinted edition: _z9781071617786
776	0	8	_iPrinted edition: _z9781071617809
776	0	8	_iPrinted edition: _z9781071617816
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1779-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI