000			04880nam a2200409 i 4500
999			_c396507 _d396507
001			396507
003			ES-MaUEC
005			20230707144159.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			211001s2022 xxu o |||| 0|eng d
020			_a9781071616895
024	7		_a10.1007/978-1-0716-1689-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .P4 _b2022 EB
245	0	0	_aPeptide Macrocycles : _bMethods and Protocols _cedited by Matthew B. Coppock, Alexander J. Winton
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XII, 469 páginas) _b106 ilustraciones, 83 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2371
505	0		_aThree Methods for Peptide Cyclization via Lactamization -- Solid-Phase Peptide Cyclization with Two Disulfide Bridges -- Peptide Macrocyclization through Palladium-Catalyzed Late-Stage C-H Activation -- Three Methods for the Solution Phase Synthesis of Cyclic Peptides -- Computational Design of Structured and Functional Peptide Macrocycles -- On-Resin Peptide Cyclization Using the 3-4-Amino-(Methylamino)Benzoic Acid (MeDbz) Linker -- Scalable and Efficient in planta Biosynthesis of Sunflower Trypsin Inhibitor-1 (SFTI) Peptide Therapeutics -- Ligation, Macrocyclization, and Simultaneous Functionalization of Peptides by Multicomponent Reactions (MCR) -- Diels-Alder Cycloadditions for Peptide Macrocycle Formation -- Protein Catalyzed Capture (PCC) Agents for Antigen Targeting -- Preparation of Bacterial Cell-Surface Displayed Semisynthetic Cyclic Peptides -- The Discovery of Peptide Macrocycle Rescuers of Pathogenic Protein Misfolding and Aggregation by Integrating SICLOPPS Technology and Ultra-Throughput Screening in Bacteria -- In Vitro Selection of Thioether-Closed Macrocycle Peptide Ligands by Means of the RaPID System -- MOrPH-PhD: A Phage Display System for the Functional Selection of Genetically Encoded Macrocyclic Peptides -- Discovery of Antimicrobial Peptide Macrocycles Through Bacterial Display -- Assessing the Cellular Uptake, Endosomal Escape, and Cytosolic Entry Efficiencies of Cyclic Peptides -- In silico Analysis of Peptide Macrocycle-Protein Interactions -- Binding Characterization of Cyclic Peptide Ligands to Target Proteins and Chemical Epitopes using ELISA and Fluorescence Polarization Assays -- Determining the Binding Kinetics of Peptide Macrocycles using Bio-Layer Interferometry (BLI) -- Different Approaches to Cyclize a Cell-Penetrating Peptide and to Tether Bioactive Payloads -- Helical Stabilization of Peptide Macrocycles by Stapled Architectures -- Development and Characterization of Light Responsive Peptide Macrocycles -- Hydrogel Formation with Enzyme-Responsive Cyclic Peptides -- Cyclization and Self-Assembly of Cyclic Peptides. .
520			_aThis volume explores the latest techniques and strategies used to study the field of peptide macrocycles. The chapters in this book ae organized into four parts: macrocycles synthesis, combinational library synthesis and screening, macrocycle characterization, and unique applications. Part One looks at a variety of peptide cyclization methodologies, and Part Two describes methods for the creation of peptide macrocycles libraries and their subsequent screening against biological targets of interest. Part Three discusses the study and characterization of peptide macrocycle-target interactions, and Part Four introduces unique applications for peptide macrocycles, from higher-order structure formation to post-synthetic functional modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Peptide Macrocycles: Methods and Protocols is a valuable resource for both novice and expert researchers looking to learn more about this developing field.
988			_aSpringer_Protocols_2022
650		7	_2embne _9146243 _aPéptidos
650		7	_2embne _9138330 _aInmunología
776	0	8	_iPrinted edition: _z9781071616888
776	0	8	_iPrinted edition: _z9781071616901
776	0	8	_iPrinted edition: _z9781071616918
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1689-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eb _zSI