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008			220727s2022 xxu| o |||| 0|eng d
020			_a9781071625217
024	7		_a10.1007/978-1-0716-2521-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRB155.5 _b2022 EB
245	0	0	_aAlternative Splicing : _bMethods and Protocols _cedited by Peter Scheiffele, Oriane Mauger
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 354 páginas) _b61 ilustraciones, 57 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2537
505	0		_aAlternative Splicing in Human Biology and Disease -- Targeting Alternative Splicing for Therapeutic Interventions -- An Optimized Protocol for the Mapping of Cell Type-Specific Ribosome-Associated Transcript Isoforms from Small Mouse Brain Regions -- FACS-Based Neuronal Cell Type-Specific RNA Isolation and Alternative Splicing Analysis -- Quantitative Measurement of Alternatively Spliced RNA Isoform Levels -- Analysis of Splicing Regulation by Third-Generation Sequencing -- Computational Analysis of Alternative Splicing Using VAST-TOOLS and the VastDB Framework -- Identification and Quantification of Microexons Using Bulk and Single-Cell RNA-Seq Data -- Functional Annotation of Custom Transcriptomes -- Design, Labeling, and Application of Probes for RNA smFISH -- BaseScope™ Approach to Visualize Alternative Splice Variants in Tissue -- In Situ Imaging of mRNA Splicing Variants by SpliceRCA -- Two-Color Fluorescent Reporters for Analysis of Alternative Splicing -- Quantitative Detection of Protein Splice Variants by Selected Reaction Monitoring (SRM) Mass Spectrometry -- Probing the Interactions of Splicing Regulatory Small Molecules and Proteins with U1 snRNP Using NMR Spectroscopy -- Genome-Wide CRISPR Screening to Identify Mammalian Factors that Regulate Intron Retention -- Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins -- Probing Liquid-Liquid Phase Separation of RNA-Binding Proteins In Vitro and In Vivo -- Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals.
520			_aThis detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects.
988			_aSpringer_Protocols_2022
650		7	_2embne _9144879 _aGenética médica
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2521-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eh _zSI