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020 _a9781071624371
024 7 _a10.1007/978-1-0716-2437-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH588 .S83
_b2022 EB
245 0 0 _aEmbryonic Stem Cell Protocols
_cedited by Kursad Turksen
250 _a4th edition 2022
264 1 _aNew York, NY
_bSpringer International Publising
_c2022
300 _a1 recurso en línea (XIII, 363 páginas)
_b1 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2520
505 0 _aDissecting Molecular Phenotypes through FACS-Based Pooled CRISPR Screens -- Feeder Free Human Embryonic Stem Cell Culture under Defined Culture Conditions -- Rat Induced Pluripotent Stem Cells-Derived Cardiac Myocytes in a Cell Culture Dish -- Transient Induction and Characterization of Mouse Epiblast-Like Cells from Mouse Embryonic Stem Cells -- High-Content Image Analysis of Spatiotemporal Proliferation and Differentiation Patterns in 3D Embryoid Body Differentiation Model -- Derivation of Multipotent Neural Progenitors from Human Embryonic Stem Cells for Cell Therapy and Biomedical Applications -- Feeder Dependent/Independent Mouse Embryonic Stem Cell Culture Protocol -- ChIP-qPCR for Polycomb Group Proteins during Neuronal Differentiation of Human Pluripotent Stem Cells -- Directed Differentiation of Human Pluripotent Stem Cells into Inner Ear Organoids -- Glycolytic Profiling of Mouse Embryonic Stem Cells (mESCs) -- Replating Protocol for Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes -- Hematopoietic Cell Isolation by Antibody-Free Flow Cytometry in the Zebrafish Embryo -- Human Trophectoderm Spheroid Derived from Human Embryonic Stem Cells -- Embryoid Bodies-Based Multilineage Differentiation of Human Embryonic Stem Cells Grown on Feeder-Free Conditions -- A Simple, Rapid, and Cost-effective Method for Loss-of-Function Assays in Pluripotent Cells -- Embryoid Body Formation from Mouse and Human Pluripotent Stem Cells for Transplantation to Study Brain Microenvironment and Cellular Differentiation -- A Modified SMART-Seq Method for Single Cell Transcriptomic Analysis of Embryoid Body Differentiation -- Osteogenic Differentiation from Mouse Embryonic Stem Cells -- Murine Embryonic Stem Cell Culture, Self-Renewal, and Differentiation -- Mouse Embryonic Stem Cell Culture in Serum-Containing or 2i Conditions -- Directed Differentiation of Mouse Embryonic Stem Cells to Mesoderm, Endoderm, and Neuroectoderm Lineages -- Accessing the Human Pluripotent Stem Cell Translatome by Polysome Profiling -- Genome Engineering Human ESCs or iPSCs with Cytosine and Adenine Base Editors -- Proteomic Analysis of Human Neural Stem Cells Differentiation by SWATH-MS.
520 _aThis fully updated volume explores improved and extended protocols for embryonic stem cell (ESC) research, provided with the most direct and informative methodologies currently available. The book examines how these models for cell lineage and differentiation studies have continued to mature into a critical research workhorse. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Embryonic Stem Cell Protocols, Fourth Edition serves as an ideal resource for researching mining the depths of the ESC field.
988 _aSpringer_Protocols_2022
650 7 _2embne
_9160528
_aCélulas madre
776 0 8 _iPrinted edition:
_z9781071624364
776 0 8 _iPrinted edition:
_z9781071624388
776 0 8 _iPrinted edition:
_z9781071624395
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2437-1
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b08/2023
_dz
_eh
_zSI