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008			220722s2022 xxu| o |||| 0|eng d
020			_a9781071624975
024	7		_a10.1007/978-1-0716-2497-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH447 _b2022 EB
245	0	0	_aSpatial Genome Organization : _bMethods and Protocols _cedited by Tom Sexton
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 333 páginas) _b52 ilustraciones, 45 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2532
505	0		_aQuantitative Chromosome Conformation Capture (3C-qPCR) -- Detection of Allele-Specific 3D Chromatin Interactions Using High-Resolution In-Nucleus 4C-Seq -- Tough Tissue Hi-C -- Mapping Mammalian 3D Genomes by Micro-C -- Targeted Chromosome Conformation Capture (HiCap) -- Assessment of Higher-Order, Multi-Way Interactions with Tri-C -- Assessing Specific Networks of Chromatin Interactions with HiChIP -- Measuring Cytological Proximity of Chromosomal Loci to Defined Nuclear Compartments with TSA-Seq -- The High-Salt Recovered Sequence-Sequencing (HRS-seq) Method: Exploring Genome Association with Nuclear Bodies -- High-Throughput Preparation of Improved Single-Cell Hi-C Libraries Using an Automated Liquid Handling System -- Simultaneous Quantification of Spatial Genome Positioning and Transcriptomics in Single Cells with scDam&T-Seq -- High-Throughput DNA FISH (hiFISH) -- Versatile CRISPR-Based Method for Site-Specific Insertion of Repeat Arrays to Visualize Chromatin Loci in Living Cells -- CLOuD9: CRISPR-Cas9-Mediated Technique for Reversible Manipulation of Chromatin Architecture -- Acute Protein Depletion Strategies to Functionally Dissect the 3D Genome.
520			_aThis detailed volume explores a variety of cutting-edge techniques used to interrogate spatial genome organization. Beginning with a section covering the vital chromosome conformation capture (3C) technique, this collection continues with chapters on targeted Hi-C approaches, sequencing-based approaches to assess nuclear environment, as well as single-cell technologies to better characterize the heterogeneity and dynamics of nuclear architectures and approaches to visualize them by microscopy. Finally, in order to be able to ask functional questions about the role of spatial chromatin organization in genomic control, the last section provides methods for acute manipulations of chromatin architecture. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Spatial Genome Organization: Methods and Protocols is an ideal resource for researchers searching for the best techniques to address their own specific research questions.
988			_aSpringer_Protocols_2022
650		7	_2embne _9162605 _aGenomas
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2497-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eh _zSI