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008			220720s2022 xxu| o |||| 0|eng d
020			_a9781071623299
024	7		_a10.1007/978-1-0716-2329-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP671 .V5 _b2022 EB
245	0	0	_aRhodopsin : _bMethods and Protocols _cedited by Valentin Gordeliy
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 363 páginas) _b66 ilustraciones, 64 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2501
505	0		_aMicrobial Rhodopsins -- Molecular Biology of Microbial Rhodopsins -- Rhodopsin-Based Optogenetics: Basics and Applications -- Searching Metagenomes for New Rhodopsins -- E. coli Expression and Purification of Microbial and Viral Rhodopsins -- Crystallization of Microbial Rhodopsins -- Crystallographic Studies of Rhodopsins: Structure and Dynamics -- Time-Resolved UV-VIS Spectroscopy of Microbial Rhodopsins -- Solid-State NMR Spectroscopy on Microbial Rhodopsins -- FTIR and Raman Spectroscopy of Rhodopsins -- Single Cell Resolution Optogenetics via Expression of Soma-Targeted Rhodopsins -- Electrophysiological Characterization of Microbial Rhodopsins Transport Properties: Electrometric and ΔpH Measurements using Planar Lipid Bilayer, Collodion Film and Fluorescent Probes Approaches -- Electrophysiological Characterization of Microbial Rhodopsins by Patch-Clamp Experiments -- Molecular Optimization of Rhodopsin-Based Tools for Neuroscience Applications -- Optogenetic Studies of Mitochondria -- In Vivo and In Vitro Characterization of Cyclase and Phosphodiesterase Rhodopsins -- Optogenetic Control of Human Stem Cell-Derived Neurons.
520			_aThis volume provides readers with the latest information on the advances made in the field of rhodopsins. The chapters in this book cover topics such as new discoveries and developments; new ways to search for rhodopsins; methods to characterize the function and structure of rhodopsins on a molecular level; nano volume high throughput in meso crystallization, and fourth generation x-ray synchrotron sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Rhodopsin: Methods and Protocols is a valuable resource for any scientist and researcher interested in learning more about this developing field.
988			_aSpringer_Protocols_2022
650		7	_2embne _9540432 _aPigmentos
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2329-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eh _zSI