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008			220714s2022 xxu| o |||| 0|eng d
020			_a9781071624739
024	7		_a10.1007/978-1-0716-2473-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH631 _b2022 EB
245	0	0	_aBioluminescence : _bMethods and Protocols _nVolume 2 _cedited by Sung-Bae Kim
250			_a4th edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XV, 427 páginas) _b125 ilustraciones, 108 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2525
505	0		_aQuantitative Assessment of the Efficacy of Near-Infrared Photoimmunotherapy with Bioluminescence Imaging -- Evaluation of the Efficacy of Saracatinib-Loaded Nanoparticles in Lymphatic Metastases of HNSCC with the Aid of Bioluminescence Imaging -- Reactive Oxygen Species-Responsive and Self-Illuminating Nanoparticles for Inflammation and Tumor Imaging -- Antibacterial Activity Evaluation of ZnO, CuO, and TiO2 Nanoparticles in Solution and Thin Films -- BRET-Based Dual-Color (Visible/Near-Infrared) Molecular Imaging Using a Quantum Dot/EGFP-Luciferase Conjugate -- Polyhistidine-Tag-Enabled Conjugation of Quantum Dots and Enzymes to DNA Nanostructures -- Real-Time Quantification of Cell Internalization Kinetics by Bioluminescent Probes -- Quantitative Imaging of Retinoic Acid Activities in Living Mammalian Cells -- Bioluminescence-Based Complementation Assay to Correlate Conformational Changes in Membrane-Bound Complexes with Enzymatic Function -- The NanoBiT-Based Homogenous Ligand-Receptor Binding Assay -- Monitoring Hippo Signaling Pathway Activity Using a Luciferase-Based Large Tumor Suppressor (LATS) Biosensor -- Screening of Protein-Protein Interaction Modulators Using BRET‑Based Technology -- TRUPATH: An Open-Source Biosensor Platform for Interrogating the GPCR Transducerome -- MERLIN: A BRET-Based Proximity Biosensor for Studying Mitochondria­-ER Contact Sites -- Single Cell NanoBRET Imaging with Green-Range Halo-Tag Acceptor -- Method for Measuring Bioactive Molecules in Blood by a Smartphone Using Bioluminescent Ratiometric Indicators -- BRET Sensors for Imaging Membrane Integrity of Microfluidically Generated Extracellular Vesicles -- In Vivo Assessment of Protein-Protein Interactions Using BRET Assay -- Live Cell Imaging of ATP Dynamics in Plant Cells -- Bioluminescence Resonance Energy Transfer for Global DNA Methylation Quantification -- In Vivo Bioluminescent Imaging of Bone Marrow Derived-Mesenchymal Stem Cells in Mice -- In Vivo Imaging of Oxidative and Hypoxic Stresses in Mice Model of Amyotrophic Lateral Sclerosis -- ATP Sensing Paper with Smartphone Bioluminescence-Based Detection -- Organ Bioluminescence Imaging under Machine Perfusion Setting for Assessing Quality of Harvested Organ Preservation -- Time-Lapse Bioluminescence Imaging of Hes7 Expression In Vitro and Ex Vivo -- Bioluminescence-Optogenetics: A Practical Guide -- Applications of Bioluminescence-Optogenetics in Rodent Models -- One-Channel Microsliding Luminometer for Quantifying Low Energy Bioluminescent Lights -- Compact Eight-Channel Light Sensing System for Bioassays -- Characterization of Firefly Flashes at Various Temperatures in Different Wavelength Regions -- Bioluminescent Monitoring of Circadian Rhythms in Isolated Mesophyll Cells of Arabidopsis at Single-Cell Level -- Exploring Phylogenetic Relationships and Divergence Times of Bioluminescent Species Using Genomic and Transcriptomic Data.
520			_aThis detailed collection explores recent advances in molecular imaging techniques involving bioluminescence, currently employed in biolaboratories around the world. Volume 2 delves into techniques for heterogeneous conjugates, protein fragment-complementation assays, BRET-based imaging, as well as instrumentation and software. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Bioluminescence: Methods and Protocols, Fourth Edition, Volume 2 presents practical guidance for researchers and technical staff on how to proceed with bioluminescence studies in their laboratories.
988			_aSpringer_Protocols_2022
650		7	_2embne _9165668 _aFisiología celular _vManuales de laboratorio
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2473-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eh _zSI