000			05597nam a22003615i 4500
999			_c396494 _d396494
001			396494
003			ES-MaUEC
005			20240122130114.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			220712s2022 xxu| s |||| 0|eng d
020			_a9781071624531
024	7		_a10.1007/978-1-0716-2453-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH631 _b2022 EB
245	0	0	_aBioluminescence : _bMethods and Protocols, Volume 1 _cedited by Sung-Bae Kim
250			_a4th edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XVI, 474 páginas) _b130 ilustraciones, 108 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2524
505	0		_aGene Cloning and Functional Analysis of the Luciferase from Luminous Syllids of the Genus Odontosyllis -- Synthetic Coelenterazine Derivatives and Their Application for Bioluminescence Imaging -- Visible Light Bioluminescence Imaging Platform for Animal Cell Imaging -- Biosynthesis-Inspired Deracemizative Production of D-Luciferin In Vitro by Combining Luciferase and Thioesterase -- Production of Metridia Luciferase in Native Form by Oxidative Refolding from E. coli Inclusion Bodies -- Production of Copepod Luciferases via Baculovirus Expression System -- Molecular Tension Probe for In Vitro Bioassays -- Optimized Loop-Mediated Amplification (LAMP) Allows Single Copy Detection Using Bioluminescent Assay in Real Time (BART) -- A Simple and Rapid Bioluminescence-Based Functional Assay of Organic Anion Transporter 1 as a d-Luciferin Transporter -- A Simple Bioluminescent Assay for the Screening of Cytotoxic Molecules against the Intracellular Form of Leishmania infantum -- A Simple, Robust, and Affordable Bioluminescent Assay for Drug Screening against Infective African Trypanosomes -- Imaging of Autonomous Bioluminescence Emission from Single Mammalian Cells -- Rapid Single-Cell Detection of Beer-contaminating Lactic Acid Bacteria Using Bioluminescence/Rapid Microbe Detection -- Bioluminescence of Aliivibrio fischeri in Artificial Seawater and Its Application in Fungicide Sensing -- A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor -- Design of an Intron-Retained Bioluminescence Reporter and Its Application in Imaging of Pre-mRNA Splicing in Living Subjects -- Generation of Bi-Reporter Expressing Tri-Segmented Arenavirus -- Bioluminescent and Fluorescent Reporter-Expressing Recombinant SARS-CoV-2 -- Generation, Characterization, and Applications of Influenza A Reporter Viruses -- Optimized Aequorin Reconstitution Protocol to Visualize Calcium Ion Transients in the Heart of Transgenic Zebrafish Embryos In Vivo -- Quantification and Imaging of Exosomes via Luciferase-Fused Exosome Marker Proteins: ExoLuc System -- Bioluminescent Tracking of Human Induced Pluripotent Stem Cells In Vitro and In Vivo -- Noninvasive In Vivo Tracking of Mammalian Cells Stably Expressing Firefly Luciferase -- Bioluminescence Imaging for Evaluation of Antitumor Effect In Vitro and In Vivo in Mice Xenografted Tumor Models -- Detection of Spontaneous Bone Metastases of Solid Human Tumor Xenografts in Mice -- In Vivo Imaging Analysis of an Inner Ear Drug Delivery in Mice: Comparison of Inner Ear Drug Concentrations Over Time -- Protocols for the Evaluation of a Lymphatic Drug Delivery System Combined with Bioluminescence to Treat Metastatic Lymph Nodes -- In Vivo Bioluminescent Imaging of Rabies Virus Infection and Evaluation of Antiviral Drug -- Imaging Infection by Vector-Borne Protozoan Parasites Using Whole-Mouse Bioluminescence -- Longitudinal Tracing of Lyssavirus Infection in Mice via In Vivo Bioluminescence Imaging -- Dual-Luciferase-Based Fast and Sensitive Detection of Malaria Hypnozoites for the Discovery of Anti-Relapse Compounds -- Synthetic Assembly DNA Cloning of Multiplex Hextuple Luciferase Reporter Plasmids -- Multiplex Hextuple Luciferase Assaying -- Molecular Imaging of Tumor Progression and Angiogenesis by Dual Bioluminescence.
520			_aThis detailed collection explores recent advances in molecular imaging techniques involving bioluminescence, currently employed in biolaboratories around the world. Volume 1 delves into techniques for the establishment of luciferins and luciferases, basic in vitro and in vivo applications, as well as protocols on multiplex imaging platforms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Bioluminescence: Methods and Protocols, Fourth Edition, Volume 1 presents practical guidance for researchers and technical staff on how to proceed with bioluminescence studies in their laboratories.
988			_aSpringer_Protocols_2022
650		7	_2embne _9165668 _aFisiología celular _vManuales de laboratorio
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2453-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eh _zSI