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008			220712s2022 xxu| o |||| 0|eng d
020			_a9781071622094
024	7		_a10.1007/978-1-0716-2209-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH509 _b2022 EB
245	0	0	_aMembrane Trafficking : _bMethods and Protocols _cedited by Jingshi Shen
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIV, 407 páginas) _b91 ilustraciones, 82 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2473
505	0		_aCharacterizing Membrane Traffic in the Early Secretory Pathway Using the RUSH Retention System -- Monitoring Intra-Golgi Transport with Acute Spatiotemporal Control of a Synthetic Cargo -- Imaging Secretory Granule Budding from the Trans-Golgi Network Using Retention Using Selective Hook (RUSH) -- Spatial and Temporal Control of Protein Secretion with Light -- Generation of Endogenously Tagged Membrane Trafficking Regulators Using CRISPR Genome Editing -- Determine the Function of the Exocyst in Vesicle Tethering by Ectopic Targeting -- Imaging Insulin Granule Dynamics in Human Pancreatic β-Cells Using Total Internal Reflection Fluorescence (TIRF) Microscopy -- Monitoring Synaptic Exocytosis Using a Whole-Cell Patch Clamp Technique -- Live-Cell Super-Resolution Imaging of Retrograde Axonal Trafficking Using Pulse-Chase Labeling in Cultured Hippocampal Neurons -- Electron Tomographic Methods for Studying Organelles of the Murine Chemical Synapse -- In Vitro Reconstitution Studies of SNAREs and Their Regulators Mediating GLUT4 Vesicle Fusion -- Imaging Single Vesicle Exocytosis with Total Internal Reflection Fluorescence Microscopy (TIRFM) -- Localizing Proteins on Single Trafficking Organelles in 3D with Semisynthetic Gold Labeling and Platinum Replica Electron Microscopy -- Endocytosis Assays Using Cleavable Fluorescent Dyes -- Reconstituting and Purifying Assembly Intermediates of Clathrin Adaptors AP1 and AP2 -- Monitoring Endosomal Cargo Retrieval to the Trans-Golgi Network by Microscopic and Biochemical Approaches -- Measuring Plasma Membrane Recycling Using Microscopic and Biochemical Approaches -- Light Scattering Techniques to Assess Self-Assembly and Hydrodynamics of Membrane Trafficking Proteins -- Measurement of Lysosome Positioning by Shell Analysis and Line Scan -- Optogenetic Control of Membrane Trafficking Using Light-Activated Reversible Inhibition by Assembly Trap of Intracellular Membranes (IM-LARIAT) -- Generating Custom Pooled CRISPR Libraries for Genetic Dissection of Biological Pathways -- Studying Unconventional Secretion of Misfolded Proteins in Cultured Cells and Primary Neurons -- Single-Molecule Optical Tweezers Study of Protein-Membrane Interactions -- Generation of Hybrid Extracellular Vesicles by Fusion with Functionalized Liposomes -- Quantitative Measurement of Extracellular Vesicle Content Delivery within Acceptor Cells.
520			_aThis detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to in vitro biochemical and biophysical assays. Covering virtually all the major trafficking branches, the book delves into the exocytic pathway, which focuses on cargo transport from the ER to the Golgi, through the Golgi cisternae, and to the plasma membrane and the extracellular space; the endocytic pathway, which includes cargo endocytosis, endosomal recycling, and lysosomal degradation; as well as emerging topics beyond the conventional exocytic and endocytic pathways. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Trafficking: Methods and Protocols provides techniques with broad applications as an ideal guide for junior researchers new to membrane trafficking as well as established membrane biologists seeking to expand their research programs.
988			_aSpringer_Protocols_2022
650		7	_2embne _9142266 _aMembranas celulares
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2209-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eh _zSI