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008			220707s2022 xxu| o |||| 0|eng d
020			_a9781071623800
024	7		_a10.1007/978-1-0716-2380-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP623 _b2022 EB
245	0	0	_apiRNA : _bMethods and Protocols _cedited by Nicholas F. Parrish, Yuka W. Iwasaki
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XII, 397 páginas) _b83 ilustraciones, 73 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2509
505	0		_aFunctional Analysis of Individual piRNAs in Aedes aegypti Cells and Embryos Using Antisense Oligonucleotides -- CRISPR-Mediated Genome Engineering in Aedes aegypti -- PIWI-Directed DNA Elimination for Tetrahymena Genetics -- Planarian PIWI-piRNA Interaction Analysis Using Immunoprecipitation and piRNA-Sequencing -- Isolation and Processing of Bovine Oocytes for Small RNA Sequencing -- 3D Imaging and In Situ Hybridization for Uncovering the Functions of MicroRNA in Rice Anther -- Cloning, Sequencing, and Linkage Analysis of piRNAs -- Drosophila Genetic Resources for Elucidating piRNA Pathway -- Generation of Stable Drosophila Ovarian Somatic Cell Lines Using the piggyBac System -- Whole Mount RNA FISH Combined with Immunofluorescence for Analysis of the Telomeric Ribonucleoproteins in the Drosophila Germline -- CRISPR-Mediated Activation of Transposable Elements in Embryonic Stem Cells -- Method for Evaluating Effects of Non-Coding RNAs on Nucleosome Stability -- Revisiting the Glass Treatment for Single Molecule Analysis of ncRNA Function -- Low Input Genome-Wide DNA Methylation Analysis with Minimal Library Amplification -- Solid-Support Directional (SSD) RNA-Seq as a Companion Method to CLIP-Seq -- UPA-Seq-Based Search Method for Functional lncRNA Candidates -- Large-Scale Analysis of RNA-Protein Interactions for Functional RNA Motif Discovery Using FOREST -- Computational Methods for the Discovery and Annotation of Viral Integrations -- Bioinformatics Approaches for Determining the Functional Impact of Repetitive Elements on Non-Coding RNAs -- Extending and Running the Mosquito Small RNA Genomics Resource Pipeline -- Preparation of Non-Overlapping Transposable Elements (TE) Annotation by Interval Tree -- Statistical Thermodynamics Approach for Intracellular Phase Separation.
520			_aThis detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several bioinformatic and biophysical methods related to piRNA studies, consistent with the increasing importance of high-throughput sequencing and computational methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, piRNA: Methods and Protocols serves as an ideal guide for researchers seeking to elucidate the numerous mysteries of this area of multicellular biology.
988			_aSpringer_Protocols_2022
650		7	_2embne _9144940 _aARN
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2380-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eh _zSI