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008			220703s2022 xxu| o |||| 0|eng d
020			_a9781071623992
024	7		_a10.1007/978-1-0716-2399-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR151 _b2022 EB
245	0	0	_aYeast Metabolic Engineering : _bMethods and Protocols _cedited by Valeria Mapelli, Maurizio Bettiga
250			_a2nd edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 295 páginas) _b51 ilustraciones, 32 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2513
505	0		_aAn Overview on Selectin Marker Genes for Transformation of Saccharomyces cerevisiae -- High Efficiency Plasmid DNA Transformation in Yeast -- CasPER, A CRISPR/Cas9-Based Method for Directed Evolution in Genomic loci in Saccharomyces cerevisiae -- Fluorescence Activated Cell Sorting as a Tool for Recombinant Strain Screening -- Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology -- Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris -- Competent Cell Preparation and Transformation of Pichia pastoris -- CRISPR/Cas9 Tool Kit for Efficient and Targeted Insertion/Deletion Mutagenesis of the Komagataella phaffii (Pichia pastoris) Genome -- Plasmid-Based Gene Knockout Strategy with Subsequent Marker Recycling in Pichia pastoris -- Engineering of Promoters for Gene Expression in Pichia pastoris -- Molecular Tools for Leveraging the Potential of the Acid Tolerant Yeast Zygosaccharomyces bailii as Cell Factory -- Golden Gate Multi-Genes Assembly Method for Yarrowia lipolytica -- A Universal Gene Expression System for Novel Yeast Species -- Dynamic Feeding of Pichia pastoris -- Automated Evolutionary Engineering of Yeasts -- Reconstruction of Genome-Scale Metabolic Model of Hansenula polymorpha using RAVEN.
520			_aThis second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the "universal expression system" that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.
988			_aSpringer_Protocols_2022
650		7	_2embne _9666009 _aLevaduras
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2399-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eh _zSI