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008			220701s2022 xxua o |||| 0|eng d
020			_a9781071624098
024	7		_a10.1007/978-1-0716-2409-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH671 _b2022 EB
245	0	0	_aNeuronal Cell Death : _bMethods and Protocols _cedited by Arezu Jahani-Asl
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XII, 357 páginas) _b78 ilustraciones, 68 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2515
505	0		_aMonitoring Mitochondrial Respiration in Mouse Cerebellar Granule Neurons -- Examining Mitochondrial Morphology in Mouse Brains -- Investigating Microglial Ultrastructural Alterations and Intimate Relationships with Neuronal Stress, Dystrophy, and Degeneration in Mouse Models of Alzheimer's Disease -- Performing Single Cell Clonal Analysis in the Mouse Brain using Mosaic Analysis with Double Markers (MADM) -- Combination of Endothelin-1 (ET-1) and L-NAME to Induce Murine Focal Murine Cortical Stroke with Persistent Sensorimotor Deficit -- Modeling Axonal Degeneration Using Motor Nerve Organoids -- Monitoring Autophagy in Neural Stem and Progenitor Cells -- Direct FACS Isolation of Neural Stem/Progenitor Lineages from the Adult Brain -- Analysis of mRNA Dynamics using RNA Sequencing Data -- Assessment of Dopaminergic Neurodegeneration in Mice -- Super-Resolution Imaging of Cytoskeletal Networks in Fixed Brain Tissue -- Spinal Cord Injury in the Mouse using the Infinite Horizons Spinal Cord Impactor -- Immuno-MALDI-MS for Accurate Quantitation of Targeted Peptides from Volume-Restricted Samples -- Three-Dimensional Atlas of the Human Amygdala Subnuclei Constructed using Immunohistochemical and Ultra-High Field Magnetic Resonance Imaging Data -- Using Single-Molecule Fluorescence Microscopy to Uncover Neuronal Vulnerability to Protein Damage -- Autopsy Human Brain Dissection Protocol for Common Age-Related Neurodegenerative Disorders -- In Vitro Brain Organoids and Computational Models to Study Cell Death in Brain Diseases -- Isolation of Mouse Embryonic Neural Stem Cells and Characterization of Neural Stem Markers by Flow Cytometry -- Assessment of Neuronal Cell Death in Caenorhabditis elegans -- Emerging Methods in Modeling Brain Development and Disease with Human Pluripotent Stem Cells -- Isolation of Adult Mouse Neural Stem Cells and Assessment of Self-Renewal by ELDA.
520			_aThis volume covers comprehensive methods on ways to assess structural and ultrastructural changes in the mitochondria, cytoskeleton, and microglia using state-of-the-art microscopy techniques including super-resolution imaging, electron microscopy, and ultra-high field MRI. The chapters in this book cover topics such as analysis of neurodegeneration in the post-mortem characterization of preclinical animal models, in vivo modeling in cell death in different model systems and brain organoids, single cell clonal analysis using Mosaic Analysis with Double Markers in genetic mouse models, and genome and proteomic methods for analysis of mRNA dynamics and quantitation of targeted peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Neuronal Cell Death: Methods and Protocols is a valuable resource for any scientist and researcher interested in learning more about this developing field.
988			_aSpringer_Protocols_2022
650		7	_2embne _9157983 _aApoptosis _vManuales de laboratorio
650		7	_2embne _9160237 _aMuerte celular _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071624081
776	0	8	_iPrinted edition: _z9781071624104
776	0	8	_iPrinted edition: _z9781071624111
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2409-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eb _zSI