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008			220701s2022 xxua o |||| 0|eng d
020			_a9781071623848
024	7		_a10.1007/978-1-0716-2384-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQD415.3 _b2022 EB
245	0	4	_aThe P2X7 Receptor : _bMethods and Protocols _cedited by Annette Nicke
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIV, 394 páginas) _b93 ilustraciones, 81 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2510
505	0		_aHow Structural Biology Has Directly Impacted Our Understanding of P2X Receptor Function and Gating -- Agonists, Antagonists, and Modulators of P2X7 Receptors -- Modulation of Cell Energy Metabolism by the P2X7 Receptor -- Fully Flexible Ligand Docking for the P2X7 Receptor Using ROSIE -- Preparation of the Murine Anti-Human P2X7 Receptor Monoclonal Antibody (Clone L4) -- Development of Antibody and Nanobody Tools for P2X7 -- Enhanced Transduction of P2X7-Expressing Cells with Recombinant rAAV Vectors -- Immunofluorescence Staining of P2X7 Receptors in Whole-Mount Myenteric Plexus Preparations -- Established Protocols for cRNA Expression and Voltage-Clamp Characterization of the P2X7 Receptor in Xenopus laevis Oocytes -- A Simplified Protocol to Incorporate the Fluorescent Unnatural Amino Acid ANAP into Xenopus laevis Oocyte-Expressed P2X7 Receptors -- Using Whole-Cell Electrophysiology and Patch Clamp Photometry to Characterize P2X7 Receptor Currents -- Using Symmetrical Organic Cation Solutions to Study P2X7 Ion Permeation -- Methods for Studying Cholesterol-Dependent Regulation of P2X7 Receptors -- Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry -- Characterization of P2X7 Receptors in Human Blood Cells -- Real-Time Flow Cytometry as a Tool to Monitor Cellular Consequences of P2X7 Activation in Multiple Cell Populations Mixed in a Single FACS Tube -- Administration of P2X7 Receptor Blockers in Oncological Experimental Models -- Humanized Mouse Model to Study the P2X7 Receptor in Graft-Versus-Host Disease -- Analysis of P2X7-Induced Neuronal Branching -- Studying the Role of P2X7 Receptor in Axonal Growth Using In Utero Electroporation Technique -- Analyzing the Role of the P2X7 Receptor in Epilepsy.
520			_aThis detailed volume covers diverse aspects of P2X7 receptor analysis, ranging from its molecular structure to related pharmacological and immunological tools, via its analysis in heterologous expression systems as well as assays using primary cells and whole animal models. After three introductory chapters that focus on its structure, ligands, and physiological functions, the book details the generation of antibody and nanobody tools for P2X7 receptors, provides protocols for the analysis of expressed P2X7 receptors with a focus on their electrophysiological analysis, as well as protocols for the investigation of P2X7 down-stream signaling in immune cells by flow cytometry. Mouse models and procedures suited to investigate P2X7-mediated effects in other primary cells and in vivo are also explained. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The P2X7 Receptor: Methods and Protocols is a valuable reference not only for the growing community fascinated by this unusual ion channel but also for a broad readership interested in ion channels or purinergic receptors.
988			_aSpringer_Protocols_2022
650		7	_2embne _9138639 _aBioquímica _vManuales de laboratorio
650		7	_2embne _9138330 _aInmunología _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071623831
776	0	8	_iPrinted edition: _z9781071623855
776	0	8	_iPrinted edition: _z9781071623862
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2384-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eb _zSI