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008			220630s2022 xxua o |||| 0|eng d
020			_a9781071624036
024	7		_a10.1007/978-1-0716-2403-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP106.6 _b2022 EB
245	0	0	_aVasculogenic Mimicry : _bMethods and Protocols _cedited by Emily Marques dos Reis, Fernanda Berti
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (X, 179 páginas) _b38 ilustraciones, 31 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2514
505	0		_aVasculogenic Mimicry - An Overview -- In vitro Models to Study Angiogenesis and Vasculature -- In vitro Tube Formation Assays in Matrigel -- Vasculogenic mimicry in a 3D model in vitro -- Cancer Cell Spheroids as a 3D Model for Exploring the Pathobiology of Vasculogenic Mimicry -- A Three-Dimensional Culture-Based Assay to Detect Early Stages of Vasculogenic Mimicry in Ovarian Cancer Cells -- Co-Immunoprecipitation of Protein Complexes from Different Subcellular Compartments in Vasculogenic Mimicry Studies -- In vivo Models to Evaluate Antitumor Drugs Effect on Vasculogenic Mimicry -- Patient derived xenograft models for studying vascular mimicry in melanoma in vivo -- Histological evaluation of long-term collagen I culture -- Histochemical Staining of Vasculogenic Mimicry -- Identification of Vasculogenic Mimicry in Histological Samples -- Evaluation of Pharmaceutical Inhibition of Vasculogenic Mimicry in vitro -- Immunohistochemistry for VM markers -- Confocal laser microscopy for VM analysis with DAPI and phalloidin staining -- Building Mathematical Models for Vascular Growth and Inhibition. .
520			_aThis volume provides detailed protocols for the identification and understanding of vasculogenic mimicry process in vitro and in vivo, in addition to protocols for microscopy and histology. Chapters guide readers through different materials, commercial and homemade scaffolds, Matrigel, cancer spheroids, 3D tissue constructs, vasculogenic processes, and mathematical model building. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Vasculogenic Mimicry: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .
988			_aSpringer_Protocols_2022
650		7	_2embne _9164656 _aNeovascularización _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071624029
776	0	8	_iPrinted edition: _z9781071624043
776	0	8	_iPrinted edition: _z9781071624050
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2403-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eb _zSI