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008			220513s2022 xxua o |||| 0|eng d
020			_a9781071621110
024	7		_a10.1007/978-1-0716-2111-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR399 _b2022 EB
245	0	0	_aSARS-CoV-2 : _bMethods and Protocols _cedited by Justin Jang Hann Chu, Bintou Ahmadou Ahidjo, Chee Keng Mok
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XVI, 478 páginas) _b76 ilustraciones, 64 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2452
505	0		_aEvolution and Epidemiology of SARS-CoV-2 Virus -- Molecular Epidemiology of SARS-CoV-2 by Sequencing -- Advanced Genetic Methodologies in Tracking Evolution and Spread of SARS-CoV-2 -- Antigen-Based Point of Care Testing (POCT) for Diagnosing SARS-CoV-2: Assessing Performance -- Diagnostic Method for COVID-19 Using Sugar Chain-Immobilized Nanoparticles and Saliva Specimens -- Detection and Quantification of SARS-CoV-2 by Real-Time RT-PCR Assay -- Immunofluorescent Antibody Techniques in the Diagnosis of SARS-CoV-2 Infection in Humans -- Propagation and Quantification of SARS-CoV-2 -- Quantification of Infectious SARS‐CoV‐2 by the 50% Tissue Culture Infectious Dose Endpoint Dilution Assay -- One-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification -- Profiling SARS-CoV-2 Infection by High-Throughput Shotgun Proteomics -- Detection of SARS-CoV-2 by Mass Spectrometry -- Bioinformatics-Based Approaches to Study Virus-Host Interactions During SARS-CoV-2 Infection -- Human Nasal Epithelial Cells (hNECs) Generated by Air-Liquid Interface (ALI) Culture as a Model System for Studying the Pathogenesis of SARS-CoV-2 -- Animal Models of COVID-19: Nonhuman Primates -- Animal Models of COVID-19: Transgenic Mouse Model -- Immunohistochemical Detection of SARS-CoV-2 Antigens, by Single and Multiple Immunohistochemistry -- Measuring Neutralizing Antibodies to SARS-CoV-2 Using Lentiviral Spike-Pseudoviruses -- Antiviral Strategies Against SARS-CoV-2: A Systems Biology Approach -- Neutralization Assay for SARS-CoV-2 Infection: Plaque Reduction Neutralization Test -- Pseudovirus-Based Assays for the Measurement of Antibody-Mediated Neutralization of SARS-CoV-2 -- Cytopathic Effect (CPE)-Based Drug Screening Assay for SARS-CoV-2 -- Strengthening Biorisk Management in Research Laboratories with Security-Sensitive Biological Agents like SARS-CoV-2 -- Biorisk Management for SARS-CoV-2 Research in a Biosafety Level-3 Core Facility -- Methods of SARS-CoV-2 Inactivation.
520			_aThis detailed volume provides the increasing number of SARS-CoV-2 researchers with a useful handbook covering multidisciplinary approaches on various aspects of SARS-CoV-2 research, brought together by leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, as well as bioinformatics and proteomics approaches in cellular response studies. This comprehensive collection also covers methods in immunology, animal models, antivirals and vaccine development strategies, as well as biorisk and mitigation measurements for SARS-CoV-2 research. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that is vital for success in the lab. Practical and timely, SARS-CoV-2: Methods and Protocols serves as an ideal guide for scientists investigating this prevalent and perilous RNA virus and the novel coronavirus disease that results from it.
988			_aSpringer_Protocols_2022
650		7	_2embne _9688509 _aCoronavirus _vManuales de laboratorio
650		7	_2embne _9413113 _aVirología _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071621103
776	0	8	_iPrinted edition: _z9781071621127
776	0	8	_iPrinted edition: _z9781071621134
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2111-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eb _zSI