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008			220228s2022 xxua o |||| 0|eng d
020			_a9781071620472
024	7		_a10.1007/978-1-0716-2047-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP608 _b2022 EB
245	0	0	_aDNAzymes : _bMethods and Protocols _cedited by Gerhard Steger, Hannah Rosenbach, Ingrid Span
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XVI, 312 páginas) _b95 ilustraciones, 77 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2439
505	0		_aA Protocol for Gold Nanoparticle-Assisted Aptamer Selection for a Small Molecule Porphyrin to Develop DNAzyme -- Selection of Aptamer for N-methyl Mesoporphyrin IX to Develop Porphyrin Metalation DNAzyme -- PCR Methods for the Generation of Catalytic ssDNA -- Design of a DNAzyme: Prediction of mRNA Regions Accessible to a DNAzyme -- Fluorescence-Based Kinetic Measurements for RNA-Cleaving DNAzymes -- Stability and Activity of the 10-23 DNAzyme under Molecular Crowding Conditions -- Quantifying the Number and Affinity of Mn2+-Binding Sites with EPR Spectroscopy -- Obtaining Crystals of Nucleic Acids in Complex with the Protein U1A Using the Soaking Method -- Crystallization and Structural Determination of 8-17 DNAzyme -- Solution NMR Spectroscopy as a Tool to Study DNAzyme Structure and Function -- Molecular Modeling and Simulations of DNA and RNA: DNAzyme as a Model System -- Single-Molecule Kinetic Studies of Nucleic Acids by Förster Resonance Energy Transfer -- Chemical Dual End-Labeling of Large Ribozymes -- Spin Labeling of Long RNAs via Click Reaction and Enzymatic Ligation -- Preparation of Site-Specifically Spin-Labeled RNA by In Vitro Transcription using an Expanded Genetic Alphabet -- PELDOR Measurements on Nitroxide-Labelled Oligonucleotides -- Sensing Metal Ions with Phosphorothioate-Modified DNAzymes -- Enhancement of Activity for Peroxidase-Mimicking DNAzyme by Covalent Attachment of Hemin to G-quadruplex-Forming Oligonucleotide Using Click Chemistry -- Generation of RNA with 2', 3'-cyclic Phosphates by Deoxyribozyme Cleavage in Frozen Solutions.
520			_aThis volume explores a collection of different protocols for the analysis and characterization of DNAzymes and their functions. The topics covered in this book range from bioinformatics and molecular dynamics simulations for the study or modification of nucleic acids to the descriptions of spectroscopic, fluorescence-based, or crystallographic methods to understand the structure and function of DNAzymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, DNAzymes: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about this evolving field. .
988			_aSpringer_Protocols_2022
650		7	_2embne-DP _9146752 _aHidrolasas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071620465
776	0	8	_iPrinted edition: _z9781071620489
776	0	8	_iPrinted edition: _z9781071620496
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2047-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b08/2023 _dz _eb _zSI