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008			210925s2022 xxua o |||| 0|eng d
020			_a9781071617014
024	7		_a10.1007/978-1-0716-1701-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2022 EB
245	0	0	_aHedgehog Signaling : _bMethods and Protocols _cedited by Xiaochun Li
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 244 páginas) _b37 ilustraciones, 27 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2374
505	0		_aGeneration and Analysis of Mosaic Spinal Cord Organoids Derived from Mouse Embryonic Stem Cells -- Evaluating Hedgehog Acyltransferase Activity and Inhibition Using the Acylation-Coupled Lipophilic Induction of Polarization (Acyl-cLIP) Assay -- Analysis of Protein Cholesterylation by Biorthogonal Labeling -- An In Situ Fluorescence Assay for Cholesterol Transporter Activity of the Patched -- Imaging of the Ciliary Cholesterol Underling the Sonic Hedgehog Signal Transduction -- Studying Hedgehog Signaling during Mouse Neural Tube Development -- Measuring and Manipulating Membrane Cholesterol for the Study of Hedgehog Signaling -- The Immunofluorescence-Based Detection of Hedgehog Pathway Components in Primary Cilia of Cultured Cells -- Analysis of Dispatched Protein Processing and Sonic Hedgehog Ligand Release -- Expression, Purification, and Structure Determination of Human PTCH1-HH-N Complexes -- Characterization of Smoothened Phosphorylation and Activation -- Cell-Based Assays for Smoothened Ubiquitination and Sumoylation -- Biochemical Assays to Directly Assess Smoothened Activation by a Conformationally Sensitive Nanobody -- Studies of SMOOTHENED Activation in Cell-Free and Reconstituted Systems -- Rapid, Direct SMOOTHENED Activity Assays in Live Cells Using cAMP-Based Conformational Sensors -- Light-Induced Differentiation of Forebrain Organoids by NVOC-SAG -- NanoBRET and NanoBiT/BRET-Based Ligand Binding Assays Permit Quantitative Assessment of Small Molecule Ligand Binding to Smoothened -- Measuring Smoothened (SMO)-Mediated Activation of the Gi Protein -- Ci/Gli Phosphorylation by the Fused/Ulk Family Kinases -- A Method for Orthotopic Transplantation of Lung Cancer in Mice.
520			_aThis detailed book explores the technical breakthroughs with biophysical and cell biological approaches that have advanced the study of molecular mechanisms underlying the generation and transduction of the hedgehog (HH) signal, indicating the important role of sterols in this pathway. Within its pages, the volume examines techniques involving several key protein components in this pathway, including HH, the signaling ligand; Dispatched, a HH secretion regulator; Patched, the receptor of HH; Smoothened, the signal transducer used to transduce the HH signal across the plasma membrane; and GLI, the transcription factor to turn on HH target genes. Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Hedgehog Signaling: Methods and Protocols provides researchers in clinical and basic science with a fresh and reliable reference for analyzing HH signal transduction through cell biological, biochemical, and biophysical approaches.
988			_aSpringer_Protocols_2022
650		7	_2embne _9139861 _aProteínas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071617007
776	0	8	_iPrinted edition: _z9781071617021
776	0	8	_iPrinted edition: _z9781071617038
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1701-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b09/2023 _dz _eb _zSI