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008			220630s2022 xxu| o |||| 0|eng d
020			_a9781071623688
024	7		_a10.1007/978-1-0716-2368-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .M44 _b2022 EB
245	0	0	_aHeterologous Expression of Membrane Proteins : _bMethods and Protocols _cedited by Isabelle Mus-Veteau
250			_a3rd edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XVII, 467 páginas) _b94 ilustraciones, 78 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2507
505	0		_aSolubilization and Purification of a5b1 Integrin from Rat Liver for Reconstitution into Nanodiscs -- Membrane Proteins Production in Escherichia coli: Protocols and Rules -- Functional Overexpression of Membrane Proteins in E. coli: The Good, the Bad, and the Ugly -- Heterologous Expression of Membrane Proteins in E. coli -- Overexpression of the ABC Transporter BmrA within Intracellular Caveolæ in Escherichia coli -- Production of Membrane Proteins in Pseudomonas stutzeri -- Overproduction of Membrane-Associated, and Integrated, Proteins Using Saccharomyces cerevisiae -- Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae -- Production and Purification of a GFP-Tagged ABC Transporter CaCdr1p -- Membrane Protein Production in the Yeast, P. pastoris -- Production and Preparation of Isotopically-Labelled Human Membrane Proteins in Pichia pastoris for Fast-MAS-NMR Analyses -- Membrane Protein Production in Insect Cells -- Thromboxane A2 G Protein-Coupled Receptor Production and Crystallization for Structure Studies -- Production of Human ABC Transporters and Oligosaccharyltransferase Complexes for Structural Studies -- The Heterologous Expression and Purification of GPCRs -- Expression and Purification of the Human Thyroid-Stimulating Hormone Receptor -- Optimization of Recombinant GPCR Proteins for Biophysical and Structural Studies Using Virus-Like Particles -- Peptide Tags and Domains for Expression and Detection of Mammalian Membrane Proteins at the Cell Surface -- Tailor the Purification and Analysis of Membrane Proteins with Modular Detergents -- Detergent Alternatives: Membrane Protein Purification Using Synthetic Nanodisc Polymers -- Detergent-Free Membrane Protein Purification Using SMA Polymer -- Cell-Free Expression of GPCRs into Nanomembranes for Functional and Structural Studies -- Cell-Free Expression of Proton-Coupled Folate Transporter in the Presence of Nanodiscs -- Microscale Thermophoresis to Evaluate the Functionality of Heterologously Overexpressed Membrane Proteins in Membrane Preparations.
520			_aThis detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.
988			_aSpringer_Protocols_2022
650		7	_2embne _9671893 _aProteínas de membranas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071623671
776	0	8	_iPrinted edition: _z9781071623695
776	0	8	_iPrinted edition: _z9781071623701
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2368-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eu _zSI