000			03907nam a22003975i 4500
999			_c396477 _d396477
001			396477
003			ES-MaUEC
005			20230728101009.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			220630s2022 xxu| o |||| 0|eng d
020			_a9781071623640
024	7		_a10.1007/978-1-0716-2364-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC591 _b2022 EB
245	0	0	_aAsthma : _bMethods and Protocols _cedited by Magdalena M. Gorska
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIV, 335 páginas) _b46 ilustraciones, 36 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2506
505	0		_aModelling Asthma in Mice Using Common Aeroallergens -- Mouse Models of Respiratory Syncytial Virus Infection -- Modeling Asthma in Mice Using Rhinovirus Infection -- Mouse Model of Sendai Virus-induced Lung Disease -- Ozone-Induced Models of Airway Hyperreactivity and Epithelial Injury -- A Novel Non-Human Primate Model of Nonatopic Asthma -- Measurement of Airway Hyperresponsiveness in Mice -- Human Precision-Cut Lung Slices: Generation of and Measurement of Contractility and Relaxation of Small Airways -- Infection of Human Precision-Cut Lung Slices with the Influenza Virus -- Human Bronchial Epithelial Cell Culture Models for Cigarette Smoke and Vaping Studies -- Isolation, Ex Vivo Culture and Stimulation of Tracheal and Nasal Chemosensory Cells -- Detection, Isolation and Functional Studies of Mouse Pulmonary Group 2 Innate Lymphoid Cells -- Isolation and Characterization of Conventional and Non-Conventional Type-2 Innate Lymphoid Cells (ILC2s) from Human Peripheral Blood Mononuclear Cells (PBMCs) -- Detection of Eosinophils in Tissue Sections by Immunohistochemistry -- Targeting Eosinophils in Mouse Models of Asthma -- Detection and Isolation of Airway Mast Cell Subsets in Mouse and Human -- Isolation of Conventional Murine Lung Dendritic Cell Subsets -- Isolation and Analysis of Macrophage Subsets from the Mouse and Human Lung -- Mass Cytometry Analysis of Whole Blood Response to an Allergen -- Methods to Detect Neutrophil Extracellular Traps in Asthma -- Analysis of Airway Vagal Neurons -- Protocols to Measure Oxidative Stress and DNA Damage in Asthma.
520			_aThis volume details a collection of laboratory protocols to study asthma in mice and humans. Chapters cover animal models of asthma, methods to measure asthma-related molecules, protocols to detect, isolate, culture and stimulate cells that contribute to asthma in mice and humans, methods to deplete these cells in vivo and study responses of intact airway tissues ex vivo. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Asthma: Methods and Protocols aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.
988			_aSpringer_Protocols_2022
650		7	_2embne _9140908 _aAsma _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071623633
776	0	8	_iPrinted edition: _z9781071623657
776	0	8	_iPrinted edition: _z9781071623664
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2364-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eu _zSI