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020 _a9781071624494
024 7 _a10.1007/978-1-0716-2449-4
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQR183
_b2022 EB
245 0 0 _aEffector-Triggered Immunity :
_bMethods and Protocols
_cedited by Thomas A. Kufer, Maria Kaparakis-Liaskos
250 _a1st edition 2022
264 1 _aNew York, NY
_bSpringer International Publising
_c2022
300 _a1 recurso en línea (XI, 319 páginas)
_b49 ilustraciones, 41 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2523
505 0 _aA Brief Introduction to Effector-Triggered Immunity -- Lambda Red Recombineering in Shigella flexneri -- Using Genomic Deletion Mutants to Investigate Effector-Triggered Immunity during Legionella pneumophila Infection -- Methods of Bacterial Membrane Vesicle Production, Purification, Quantification, and Examination of their Immunogenic Functions -- Gene Gun-Mediated Transient Gene Expression for Functional Studies in Plant Immunity -- Host Induced Gene Silencing using BPMV on Soybean to Study Genes in the Soybean Rust Fungus Phakopsora pachyrhizi -- Virus-Mediated Protein Overexpression (VOX) in Monocots to Identify and Functionally Characterize Fungal Effectors -- Time-Resolved Fluorescence Microscopy Screens on Host Protein Subversion during Bacterial Cell Invasion -- Assaying RIPK2 Activation by Complex Formation -- Identification of Novel Endogenous NOD Ligands: Quantitative Analysis of Binding Affinities of NOD1 or NOD2 with Sphingosine-1-Phosphate using Microscale Thermophoresis -- A Surface Plasmon Resonance-Based Strategy to Characterize Interactions of NLR Proteins with Associated Factors -- Functional Assessment of Disease-Associated Pyrin Variants -- An Assay for the Seeding of Homotypic Pyrin Domain Filament Transitions -- Detection of Gasdermin Activation and Lytic Cell Death during Pyroptosis and Apoptosis -- Visualizing Effector Triggered Immunity in Response to Pore-Forming Toxins by Live-Cell Imaging -- Non-Apoptotic Cell Death Control of Neutrophil Extracellular Trap Formation -- Analysis of Bacteria-Triggered Inflammasome-Activation in Neutrophils by Immunoblot -- Quantifying Regulated Mitochondrial Fission in Macrophages -- Live Cell Imaging of T Cell Pyroptosis.
520 _aThis volume presents a collection of protocols to study effector-triggered immunity (ETI) in both plants and animals from eminent groups in the field. The chapters in this book cover topics such as genetic manipulation of plant and animal pathogens, host cells, and the analysis of key host responses; and techniques used for the analysis of inflammasome activation, cell death pathways, and mitochondria damage in response to pathogens. All of these topics cover a broad spectrum of immunological, biochemical, cell biological, and structural biology approaches to examine ETI. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Effector-Triggered Immunity: Methods and Protocols is a valuable resource for both expert and novice researchers who are interested in learning more about the important and developing field of ETI.
988 _aSpringer_Protocols_2022
650 7 _2embne
_9138330
_aInmunología
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781071624487
776 0 8 _iPrinted edition:
_z9781071624500
776 0 8 _iPrinted edition:
_z9781071624517
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2449-4
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b07/2023
_dz
_eu
_zSI