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020			_a9781071623763
024	7		_a10.1007/978-1-0716-2376-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC269.7 _b2022 EB
245	0	0	_aCancer Cell Biology : _bMethods and Protocols _cedited by Sherri L. Christian
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 379 páginas) _b81 ilustraciones, 68 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2508
505	0		_aOverview of the maintenance of authentic cancer cell lines -- Culturing suspension cancer cell lines -- Culture of Adherent Cancer Cell Lines -- Breast Cancer Xenograft Murine Models -- Processing and cryopreservation of blood, cancer tissues and cancer cells for viable biobanking -- Adipocyte-breast cancer cell co-culture in transwells -- A 3-dimensional co-culture method for studying interactions between adipocytes, extracellular matrix, and cancer cells -- Modelling Collective Invasion and Single-Cell Mesenchymal Invasion in Three-Dimensional Matrigel-Collagen I Cultures -- CellTrace™ Violet Flow Cytometric Assay to Assess Cell Proliferation -- Enzyme-Linked Immunosorbent Assay (ELISA) -- Flow cytometry analysis to detect lapatinib-induced modulation of constitutive and IFN-g induced HLA class I expression in HER2-positive breast cancer cells -- An end-to-end workflow for interrogating tumour infiltrating myeloid cells using mass cytometry -- Manual Immunofluorescence of Formalin-Fixed Paraffin-Embedded Human Tumour Tissues -- Western blot analysis of lapatinib-mediated inhibition of the epidermal growth factor receptor 2 (HER2) pathway in breast cancer cells -- Evaluating cell membrane localization and intracellular transport of proteins by biotinylation -- Multiplexed quantitative proteomic profiling of cancer cells and tissues using isobaric labeling-based tags -- The use of Seahorse XF assays to interrogate real-time energy metabolism in cancer cell lines -- Using CRISPR-Cas9 to dissect cancer mutations in cell Iines -- Single-cell RNA sequencing analysis using Fluidigm C1 platform for characterization of heterogeneous transcriptomes -- Sequences to Differences in Gene Expression: Analysis of RNA-Seq Data -- Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) for gene expression analyses -- Peptide-affinity isolation of extracellular vesicles and cell-free DNA from human plasma -- Characterizing Extracellular vesicles using Nanoparticle Tracking Analysis.
520			_aThis volume provides detailed methods on the mechanisms of underlying cancer cell biology. Chapters guide readers through techniques for culturing cancer cell lines, xenografts, cryopreservation of tumor cells, analyzing the co-culture of breast cancer cells, protein secretion by ELISA, flow cytometry-based, multi-parametric immunofluorescence analysis, protein expression by western blot, analysis of surface protein levels, protein recycling by biotinylation assay, and proteomics analysis by liquid chromatography-mass spectrometry. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Cancer Cell Biology: Methods and Protocols aims to provide a comprehensive set of tools for the analysis of cancer cell biology in the lab.
988			_aSpringer_Protocols_2022
650		7	_2embne _9146156 _aCélulas cancerosas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071623756
776	0	8	_iPrinted edition: _z9781071623770
776	0	8	_iPrinted edition: _z9781071623787
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2376-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eu _zSI