000			04695nam a22003975i 4500
999			_c396469 _d396469
001			396469
003			ES-MaUEC
005			20230728093434.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			220622s2022 xxu| o |||| 0|eng d
020			_a9781071623497
024	7		_a10.1007/978-1-0716-2349-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK495 .A1 _b2022 EB
245	0	0	_aCatharanthus roseus : _bMethods and Protocols _cedited by Vincent Courdavault, Sebastien Besseau
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIV, 319 páginas) _b69 ilustraciones, 59 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2505
505	0		_aRNA in situ hybridization on paraffin-sections to characterize the multicellular compartmentation of plant secondary metabolisms -- Imaging MS analysis in Catharanthus roseus -- Surface-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry (SALDI-IMS)-Based Detection of Vinca Alkaloids Distribution in the Petal of Madagascar Periwinkle -- Sample preparation, data acquisition, and data analysis for 15N-labeled and non-labeled monoterpene indole alkaloids in Catharanthus roseus -- Studying iridoid transport in Catharanthus roseus by grafting -- Pictet-Spengler reaction for the chemical synthesis of strictosidine -- Implementation of a MS/MS spectral library for Monoterpene Indole Alkaloids -- Semi-Synthesis of Bis-Indole Alkaloid (-)-Melodinine K Enabled by a Combination of Biotransformation and Chemical Synthesis -- RNA-seq analysis towards monoterpene indole alkaloid biosynthetic pathway elucidation in Catharanthus roseus -- Predicting Monoterpene Indole Alkaloid related genes from expression data with artificial neural networks -- Discovery and characterization of oxidative enzymes in monoterpenoid indole alkaloid biosynthesis -- Ancestral sequence reconstruction for exploring alkaloid evolution -- Generating an EMS mutant population and rapid mutant screening by thin layer chromatography enables the studies of monoterpenoid indole alkaloids biosynthesis in Catharanthus roseus -- TARGETing transcriptional regulation in the medicinal plant Catharanthus roseus -- Identification and Characterization of Transcription Factors Regulating Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus -- From Methylome to Integrative Analysis of Tissue-Specificity -- Tagging and capture of prenylated CaaX-proteins from plant cell cultures -- EASI transformation protocol: An Agrobacterium-mediated transient transformation protocol for Catharanthus roseus seedlings -- A rapid and efficient vacuum-based Agroinfiltration protocol for transient gene overexpression in leaves of Catharanthus roseus -- Transient gene expression in Catharanthus roseus flower petals using agroinfiltration -- De novo shoot bud induction from the Catharanthus roseus leaf explants and Agrobacterium tumefaciens mediated technique to raise transgenic plants -- Agrobacterium-mediated in planta transformation in Periwinkle.
520			_aThis volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Catharanthus roseus: Methods and Protocols aims to be a guidebook to all researchers working at characterizing alkaloid biosynthesis and more broadly specialized metabolisms.
988			_aSpringer_Protocols_2022
650		7	_2embne _9138505 _aAngiospermas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071623480
776	0	8	_iPrinted edition: _z9781071623503
776	0	8	_iPrinted edition: _z9781071623510
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2349-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eu _zSI