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008			220621s2022 xxu| o |||| 0|eng d
020			_a9781071621523
024	7		_a10.1007/978-1-0716-2152-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH362 _b2022 EB
245	0	0	_aDirected Evolution : _bMethods and Protocols _cedited by Andrew Currin, Neil Swainston
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 278 páginas) _b63 ilustraciones, 58 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2461
505	0		_aDesigning Overlap Extension PCR Primers for Protein Mutagenesis: A Programmatic Approach -- Recombination of Single Beneficial Substitutions Obtained from Protein Engineering by Computer-Assisted Recombination (CompassR) -- Non-Degenerate Saturation Mutagenesis: Library Construction and Analysis via MAX and ProxiMAX Randomization -- Antha-Guided Automation of Darwin Assembly for the Construction of Bespoke Gene Libraries -- SpeedyGenesXL An Automated, High-Throughput Platform for the Preparation of Bespoke Ultra-Large Variant Libraries for Directed Evolution -- Facile Assembly of Combinatorial Mutagenesis Libraries using Nicking Mutagenesis -- GeneORator: An Efficient Method for the Systematic Mutagenesis of Entire Genes -- Rapid Cloning of Random Mutagenesis Libraries using PTO-QuickStep -- Construction of Strong Promoters by Assembling Sigma Factor Binding Motifs -- Application of Restriction Free (RF) Cloning in Circular Permutation -- Site-Directed Mutagenesis Method Mediated by Cas9 -- Directed Evolution of Transcription Factor-Based Biosensors for Altered Effector Specificity -- A Screening Method for P450 BM3 Mutant Libraries using Multiplexed Capillary Electrophoresis for Detection of Enzymatically Converted Compounds -- Directed Evolution of Glycosyltransferases by a Single-Cell Ultrahigh-Throughput FACS-Based Screening Method -- Learning Strategies in Protein Directed Evolution.
520			_aThis volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.
988			_aSpringer_Protocols_2022
650		7	_2embne _9138470 _aEvolución _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071621516
776	0	8	_iPrinted edition: _z9781071621530
776	0	8	_iPrinted edition: _z9781071621547
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2152-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eu _zSI