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008			220610s2022 xxu| o |||| 0|eng d
020			_a9781071622698
024	7		_a10.1007/978-1-0716-2269-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP601 _b2022 EB
245	0	0	_aMultienzymatic Assemblies : _bMethods and Protocols _cedited by Haralambos Stamatis
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XII, 379 páginas) _b89 ilustraciones, 77 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2487
505	0		_aRecombinant Expression and Purification of Large Bacterial Multienzyme Assemblies for Biosynthetic Processes -- Multi-Dimensional Fluorescence Live-Cell Imaging for Glucosome Dynamics in Living Human Cells -- Mechanisms and Effects of Substrate Channelling in Enzymatic Cascades -- Structural Characterization of Multienzyme Assemblies: An Overview -- Assessing Protein Interactions for Clustering of Mitochondrial Urea Cycle Enzymes -- DNA Nano-Scaffolds for Multienzyme Systems Assembly -- Strategies for Multienzyme Assemblies -- Random and Positional Immobilization of Multi-Enzyme Systems -- Compartmentalized Immobilization of Multi-Enzyme Systems -- 3D Printed Polylactic Acid Well-Plate for Multi-Enzyme Immobilization -- Rational Design of Self-Assembling Supramolecular Protein Nanostructures Utilizing The Cucurbit[8]Uril Macrocyclic Host -- Lectin-Mediated Coimmobilization of Cascade Glycoenzymes -- Self-Assembled Multienzyme Nanostructures for Biocatalysis In Cellulo -- Nanoarmored Multi-Enzyme Cascade Catalysis -- Implementing Multi-Enzyme Biocatalytic Systems Using Nanoparticle Scaffolds -- A Four-Enzyme Nanoassembly Consisting of Hydrolases and Oxidoreductases for Multi-Step Cascade Reactions -- A Bi-Enzymatic Immobilized Nanobiocatalyst for The Biotransformation of Oleuropein to Hydroxytyrosol -- A Simple Recombinant E. coli Cell Lysate-Based Biocatalyst for ATP-Dependent Multi-Step Reactions -- CO2 to Methanol: A Highly Efficient Enzyme Cascade -- Multienzyme Catalysis in Phase-Separated Protein Condensates -- Robust and Continuous Lipase-Catalyzed Reactions in Deep Eutectic Solvents: Low Viscosity and Double CLEA-Lentikats Immobilization (CLEA-LK-Lipases) -- Enzymatic Photometric Assays for the Selective Detection of Halides.
520			_aThis volume details cutting-edge methods and protocols for the development, characterization, and applications of multienzyme assemblies. Chapters guide readers through up-to-date techniques applied for the development and emerging applications of multi-enzymatic systems for biotransformations, biosensing, molecular-scale diagnostics and bioelectronics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Multienzymatic Assemblies: Methods and Protocols aims to be useful for academics and industry professionals working in the field of biotechnology, biochemistry, chemical and biological engineering, nanobiotechnology, and biocatalysis.
988			_aSpringer_Protocols_2022
650		7	_2embne _9138640 _aEnzimas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071622681
776	0	8	_iPrinted edition: _z9781071622704
776	0	8	_iPrinted edition: _z9781071622711
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2269-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eu _zSI