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008			220608s2022 xxu| o |||| 0|eng d
020			_a9781071622018
024	7		_a10.1007/978-1-0716-2201-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .N67 _b2022 EB
245	0	0	_aNotch Signaling Research : _bMethods and Protocols _cedited by Dongyu Jia
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIII, 298 páginas) _b56 ilustraciones, 45 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2472
505	0		_aUsing the CRISPR/Cas9 System for Dissection of Functional Sites of the Notch Gene in Drosophila melanogaster -- Screening Mutants by Single Fly Genomic PCR -- Generation of Properly Folded Epidermal Growth Factor-Like (EGF) Repeats and Glycosyltransferases Enables In Vitro O-Glycosylation -- Use of FLP/FRT System to Screen for Notch Signaling Regulators in the Drosophila Wing -- High-Throughput Analysis to Identify Activators of Notch Signaling -- Artificial Notch Signaling Activation Method Using Immobilized Ligand Beads -- Mammalian NOTCH Receptors Activation and Signaling Protocols -- Somatic Clonal Analyses Using FLP/FRT and MARCM System to Understand Notch Signaling Mechanism and Its Regulation -- Analyzing the Interaction of RBPJ with Mitotic Chromatin and Its Impact on Transcription Reactivation upon Mitotic Exit -- Assessing the Roles of Potential Notch Signaling Components in Instructive and Permissive Pathways with Two Drosophila Pericardial Reporters -- Image-Based Single-Molecule Analysis of Notch-Dependent Transcription in Its Natural Context -- An Automatic Stage Identification MATLAB Tool to Reveal Notch Expression Pattern in Drosophila Egg Chambers -- Employing the CRISPR Technology for Studying Notch Signaling in the Male Gonad of Drosophila melanogaster -- Immuno-Localization of Notch Signaling in Mouse Preimplantation Embryos -- Studying the NOTCH Signaling Pathway Activation in Kidney Biopsies -- Exosomes as Carriers for Notch Molecules -- In Vivo and Ex Vivo Experimental Approach for Studying Functional Role of Notch in Pulmonary Vascular Disease -- Metastasis Model to Test the Role of Notch Signaling in Prostate Cancer -- Functional Studies of Genetic Variants Associated with Human Diseases in Notch Signaling-Related Genes Using Drosophila -- Bioinformatics Tools to Understand Notch.
520			_aThis detailed volume explores the large research area of Notch signaling studies in a variety of model organisms. The collected research methods and protocols include the dissection of Notch functional sites, Notch regulators, Notch activity reporters and analysis, roles of Notch in development and diseases, wet and dry lab tools and studies, and bioinformatics analysis. This book particularly emphasizes on the exploration of Notch roles in development and diseases, while acknowledging the importance of combining classic and novel molecular tools and methods, bioinformatics workflows and applications, and different model organisms to fully understand the subject area. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Notch Signaling Research: Methods and Protocols is an ideal guide for researchers in the field, with techniques that could potentially be applied to study the wider world of other signaling pathways as well.
988			_aSpringer_Protocols_2022
650		7	_2embne _9139861 _aProteínas _vManuales de laboratorio
650		7	_2embne _9138044 _aGenética _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071622001
776	0	8	_iPrinted edition: _z9781071622025
776	0	8	_iPrinted edition: _z9781071622032
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2201-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eu _zSI