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008			210923s2022 xxu| o |||| 0|eng d
020			_a9781071617830
024	7		_a10.1007/978-1-0716-1783-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH588 .S83 _b2022 EB
245	0	0	_aNeural Progenitor Cells : _bMethods and Protocols _cedited by Loic P. Deleyrolle
250			_a2nd edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 223 páginas) _b55 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2389
505	0		_aCulturing Mouse Fetal Neural Precursor Cells in a Free-Floating Serum-Free Condition -- Isolation and Culture of Neural Stem/Progenitor Cells from the Postnatal Periventricular Region -- Isolation and Culture of Adult Hippocampal Precursor Cells as Free-Floating Neurospheres -- Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord -- Culturing and Expansion of "Clinical Grade" Neural Stem Cells from the Fetal Human Central Nervous System -- Microglia Isolation from Neural Stem Cell-Enriched Regions -- Microglial FACS Sorting for Robust RNA Recovery for Next Generation Sequencing -- Method for Isolating Extracellular Vesicles from Human Neural Stem Cells Expanded under Neurosphere Culture -- Isolating and Culturing of Precursor Cells from the Adult Human Brain -- Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord -- Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny -- Identifying Neural Progenitor Cells in the Adult Human Brain -- Neonatal Transplant in Hypoxic Injury -- Isolation and Purification of Self Renewable Human Neural Stem Cells from iPSCs for Cell Therapy in Experimental Model of Ischemic Stroke -- Generating Cerebral Organoids from Human Pluripotent Stem Cells -- Advancing Our Understanding of Brain Disorders: Research Using Postmortem Brain Tissue -- Biovalue in Human Brain Banking: Applications and Challenges for Research in Neurodegenerative Diseases.
520			_aThis updated edition collects cutting-edge techniques used to study neural stem and progenitor cells as well as the brain microenvironment. Featuring a wide range of technological advances in the study of neural stem cells, the volume highlights the promises of stem cell-based therapeutic applications for central nervous system ailments. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neural Progenitor Cells: Methods and Protocols, Second Edition serves as an invaluable resource for the next generation of neuroscientists as they develop innovative experimental paradigms and progress toward therapeutic applications in the field of neurobiology.
988			_aSpringer_Protocols_2022
650		7	_2embne _9160528 _aCélulas madre _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071617823
776	0	8	_iPrinted edition: _z9781071617847
776	0	8	_iPrinted edition: _z9781071617854
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1783-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b07/2023 _dz _eu _zSI