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020 _a9781071624692
024 7 _a10.1007/978-1-0716-2469-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aReactive Oxygen Species in Plants
_bMethods and Protocols
_cedited by Amna Mhamdi.
250 _a1st edition 2022
264 1 _aNew York, NY
_bSpringer International Publising
_c2022
300 _a1 recurso en línea (XIV, 298 páginas)
_b53 ilustraciones, 36 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2526
505 0 _aModification of Chloroplast Antioxidant Capacity by Plastid Transformation -- Analysis of Ascorbate Metabolism in Arabidopsis under High-Light-Stress -- Genetic Manipulation of Reactive Oxygen Species (ROS) Homeostasis Utilizing CRISPR/Cas9-Based Gene Editing in Rice -- Studying Plant Stress Reactions In Vivo by PAM Chlorophyll Fluorescence Imaging -- Live Monitoring of ROS-Induced Cytosolic Redox Changes with roGFP2-Based Sensors in Plants -- Quantitative Measurement of Ascorbate and Glutathione by Spectrophotometry -- Measurement of NAD(P)H and NADPH-Generating Enzymes -- Quantitative Analysis for ROS-Producing Activity and Regulation of Plant NADPH Oxidases in HEK293T Cells -- Estimation of the Level of Abasic Sites in Plant mRNA Using Aldehyde Reactive Probe -- A Simplified Method to Assay Protein Carbonylation by Spectrophotometry -- In Vitro Biochemical Analysis of Recombinant Plant Proteins under Oxidation -- Methods to Analyze the Redox Reactivity of Plant Proteins -- Determination of ROS-Induced Lipid Peroxidation by HPLC-Based Quantification of Hydroxy Polyunsaturated Fatty Acids -- Detection of Lipid Peroxidation-Derived Free Azelaic Acid, a Biotic Stress Marker and Other Dicarboxylic Acids in Tobacco by Reversed Phase HPLC-MS under Non-Derivatized Conditions -- Determination of Reactive Carbonyl Species, which Mediate Reactive Oxygen Species Signals in Plant Cells -- Measuring Stress-Induced Changes in Defense Phytohormones and Related Compounds -- Targeted Mass Spectrometry Analysis of Protein Phosphorylation by Selected Ion Monitoring Coupled to Parallel Reaction Monitoring (tSIM/PRM) -- Quantitative Analysis of Post-Translational Modifications of Plant Histones -- Characterization of RBPome in Oxidative Stress Conditions -- Analysis of ROS-Triggered Changes in the Transcriptome.
520 _aThis detailed volume explores techniques to study reactive oxygen species (ROS) in plants and to characterize their roles in development and stress responses. Beginning with a section on strategies to induce ROS production, the book continues with methods to visualize ROS and detect changes in redox homeostasis, small-scale and targeted analyses for investigating the effects of ROS accumulation during stress on plant physiology and metabolism, as well as systems biology approaches to understand ROS functions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reactive Oxygen Species in Plants: Methods and Protocols serves as a vital resource that any researcher, and in particular young researchers, can use and adapt to further our knowledge of this dynamic area of plant science.
700 1 _aMhamdi, Amna
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781071624685
776 0 8 _iPrinted edition:
_z9781071624708
776 0 8 _iPrinted edition:
_z9781071624715
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2469-2
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2022
999 _c396448
_d396448